Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27591 | 3' | -58.1 | NC_005882.1 | + | 23067 | 0.67 | 0.389715 |
Target: 5'- -cGCcgauGAUGUCCGcGGC-CGUGACCUu -3' miRNA: 3'- gaCGuc--CUACAGGU-CCGcGCACUGGA- -5' |
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27591 | 3' | -58.1 | NC_005882.1 | + | 3680 | 0.71 | 0.195047 |
Target: 5'- -cGgAGGuauucGUCCAGGCGCG-GGCCg -3' miRNA: 3'- gaCgUCCua---CAGGUCCGCGCaCUGGa -5' |
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27591 | 3' | -58.1 | NC_005882.1 | + | 23224 | 0.72 | 0.179649 |
Target: 5'- -cGCAGGcgcUGgCCGGGCGCG-GGCCg -3' miRNA: 3'- gaCGUCCu--ACaGGUCCGCGCaCUGGa -5' |
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27591 | 3' | -58.1 | NC_005882.1 | + | 17477 | 1.07 | 0.000396 |
Target: 5'- cCUGCAGGAUGUCCAGGCGCGUGACCUu -3' miRNA: 3'- -GACGUCCUACAGGUCCGCGCACUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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