Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27592 | 3' | -54 | NC_005882.1 | + | 8929 | 0.69 | 0.519003 |
Target: 5'- --gACGGCGAUUUCGUG-CGCcUGGCc -3' miRNA: 3'- cugUGCUGCUAAAGCGCcGCGcACUG- -5' |
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27592 | 3' | -54 | NC_005882.1 | + | 7850 | 0.66 | 0.676647 |
Target: 5'- uGAgGCGAUGGggUUCGCGGaGCGUa-- -3' miRNA: 3'- -CUgUGCUGCUa-AAGCGCCgCGCAcug -5' |
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27592 | 3' | -54 | NC_005882.1 | + | 6284 | 0.7 | 0.45546 |
Target: 5'- cGACACGGCG--UUUGCGuuGCGUGGu -3' miRNA: 3'- -CUGUGCUGCuaAAGCGCcgCGCACUg -5' |
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27592 | 3' | -54 | NC_005882.1 | + | 6219 | 0.71 | 0.377779 |
Target: 5'- uGACGCGGCGGagaUUUUGCG-CGCGaaUGGCa -3' miRNA: 3'- -CUGUGCUGCU---AAAGCGCcGCGC--ACUG- -5' |
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27592 | 3' | -54 | NC_005882.1 | + | 2845 | 0.66 | 0.686799 |
Target: 5'- gGACGCGGUGAUUcggauauucgaccUgGCGGCGCGaaGCa -3' miRNA: 3'- -CUGUGCUGCUAA-------------AgCGCCGCGCacUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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