Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27595 | 3' | -55.2 | NC_005882.1 | + | 24409 | 0.68 | 0.432717 |
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Target: 5'- -gGCGGCgaagACAUGCUgGCUGacuuccgcgccaacaUcGGCGGCu -3' miRNA: 3'- uaUGCUG----UGUACGAgCGAC---------------A-CCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 25763 | 0.66 | 0.556808 |
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Target: 5'- -cGCGAUgucgcgcagGCcgGCuUCGaagGUGGCGGCc -3' miRNA: 3'- uaUGCUG---------UGuaCG-AGCga-CACCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 27600 | 0.68 | 0.469639 |
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Target: 5'- -gGCGACGCAgGC-CGCgGccGCGGCg -3' miRNA: 3'- uaUGCUGUGUaCGaGCGaCacCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 27895 | 0.66 | 0.590777 |
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Target: 5'- uUGCGaACACGUcaGCUC-CUGUacGcGCGGCg -3' miRNA: 3'- uAUGC-UGUGUA--CGAGcGACA--C-CGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 29180 | 0.67 | 0.512462 |
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Target: 5'- --cCGGCGCGccguUGCUCGC-GU-GCGGCc -3' miRNA: 3'- uauGCUGUGU----ACGAGCGaCAcCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 29331 | 0.69 | 0.409063 |
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Target: 5'- -gGCGACGaucgcgGCUCGC-GUGGCGaaGCg -3' miRNA: 3'- uaUGCUGUgua---CGAGCGaCACCGC--CG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 32137 | 0.68 | 0.448921 |
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Target: 5'- cUGCGGCcguACGUGCcugcgcgaUCGUUGUGuCGGCg -3' miRNA: 3'- uAUGCUG---UGUACG--------AGCGACACcGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 34560 | 0.69 | 0.408095 |
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Target: 5'- uUACGGCAgAUGagaUCGUUGUcgagacggcgcucGGCGGUg -3' miRNA: 3'- uAUGCUGUgUACg--AGCGACA-------------CCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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