Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27595 | 3' | -55.2 | NC_005882.1 | + | 8589 | 0.7 | 0.362467 |
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Target: 5'- -aGCGGCACGUGUggaCGCUGUcG-GGCa -3' miRNA: 3'- uaUGCUGUGUACGa--GCGACAcCgCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 8283 | 0.69 | 0.409063 |
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Target: 5'- -gAgGACGCGUGCggugccgauuUCGUcacucgcuggGUGGCGGCa -3' miRNA: 3'- uaUgCUGUGUACG----------AGCGa---------CACCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 7696 | 0.66 | 0.568078 |
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Target: 5'- --uCGAgGC--GCUCGCg--GGCGGCg -3' miRNA: 3'- uauGCUgUGuaCGAGCGacaCCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 7256 | 0.67 | 0.512462 |
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Target: 5'- uUGCGGCgaACAUGCgUCGUagUGUGGaaGCg -3' miRNA: 3'- uAUGCUG--UGUACG-AGCG--ACACCgcCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 7199 | 0.68 | 0.469639 |
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Target: 5'- cUACGACGCAUGUUCGCcgcaaUGUcGCGu- -3' miRNA: 3'- uAUGCUGUGUACGAGCG-----ACAcCGCcg -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 6643 | 0.67 | 0.505931 |
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Target: 5'- cGUGCGGCgcgccgugcgucucgGCGUGCUgCGCcaagGUuucgaaGGCGGCa -3' miRNA: 3'- -UAUGCUG---------------UGUACGA-GCGa---CA------CCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 5384 | 0.67 | 0.534472 |
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Target: 5'- -gACGGCGCcuugAUGagUUGCUG-GGCGGUa -3' miRNA: 3'- uaUGCUGUG----UACg-AGCGACaCCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 4918 | 0.67 | 0.534472 |
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Target: 5'- -cGCGcCGCu--CUCGCUGaucgaGGCGGCa -3' miRNA: 3'- uaUGCuGUGuacGAGCGACa----CCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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