Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27595 | 3' | -55.2 | NC_005882.1 | + | 7199 | 0.68 | 0.469639 |
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Target: 5'- cUACGACGCAUGUUCGCcgcaaUGUcGCGu- -3' miRNA: 3'- uAUGCUGUGUACGAGCG-----ACAcCGCcg -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 27600 | 0.68 | 0.469639 |
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Target: 5'- -gGCGACGCAgGC-CGCgGccGCGGCg -3' miRNA: 3'- uaUGCUGUGUaCGaGCGaCacCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 22368 | 0.67 | 0.490834 |
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Target: 5'- -aGCGACGC-UGCUCGCgaugGGCuaccugaagccGGCc -3' miRNA: 3'- uaUGCUGUGuACGAGCGaca-CCG-----------CCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 18284 | 0.67 | 0.501597 |
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Target: 5'- -gACGAuuCACAUGCgCGUc--GGCGGCa -3' miRNA: 3'- uaUGCU--GUGUACGaGCGacaCCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 6643 | 0.67 | 0.505931 |
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Target: 5'- cGUGCGGCgcgccgugcgucucgGCGUGCUgCGCcaagGUuucgaaGGCGGCa -3' miRNA: 3'- -UAUGCUG---------------UGUACGA-GCGa---CA------CCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 17807 | 0.67 | 0.512462 |
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Target: 5'- -aACGGCugGggcugccggUGC-CGC-GUGGUGGCg -3' miRNA: 3'- uaUGCUGugU---------ACGaGCGaCACCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 29180 | 0.67 | 0.512462 |
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Target: 5'- --cCGGCGCGccguUGCUCGC-GU-GCGGCc -3' miRNA: 3'- uauGCUGUGU----ACGAGCGaCAcCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 15520 | 0.72 | 0.273497 |
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Target: 5'- -cGCGAgCACAUGC-CGCccGUgaaGGCGGCg -3' miRNA: 3'- uaUGCU-GUGUACGaGCGa-CA---CCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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