Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27595 | 3' | -55.2 | NC_005882.1 | + | 29331 | 0.69 | 0.409063 |
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Target: 5'- -gGCGACGaucgcgGCUCGC-GUGGCGaaGCg -3' miRNA: 3'- uaUGCUGUgua---CGAGCGaCACCGC--CG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 34560 | 0.69 | 0.408095 |
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Target: 5'- uUACGGCAgAUGagaUCGUUGUcgagacggcgcucGGCGGUg -3' miRNA: 3'- uAUGCUGUgUACg--AGCGACA-------------CCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 23915 | 0.69 | 0.371486 |
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Target: 5'- cGUACGuCAgGcGUUCGagcgGUGGCGGCa -3' miRNA: 3'- -UAUGCuGUgUaCGAGCga--CACCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 12260 | 0.69 | 0.371486 |
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Target: 5'- cAUGCGAgCGCAUgGCgaugaCGCUGUcggccuauaacGGCGGCc -3' miRNA: 3'- -UAUGCU-GUGUA-CGa----GCGACA-----------CCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 8589 | 0.7 | 0.362467 |
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Target: 5'- -aGCGGCACGUGUggaCGCUGUcG-GGCa -3' miRNA: 3'- uaUGCUGUGUACGa--GCGACAcCgCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 15520 | 0.72 | 0.273497 |
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Target: 5'- -cGCGAgCACAUGC-CGCccGUgaaGGCGGCg -3' miRNA: 3'- uaUGCU-GUGUACGaGCGa-CA---CCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 14011 | 0.72 | 0.259335 |
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Target: 5'- cGUGCGGCuucCGUGCUCGCcuUGaucGCGGCg -3' miRNA: 3'- -UAUGCUGu--GUACGAGCG--ACac-CGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 19653 | 1.1 | 0.000406 |
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Target: 5'- cAUACGACACAUGCUCGCUGUGGCGGCg -3' miRNA: 3'- -UAUGCUGUGUACGAGCGACACCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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