Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27597 | 5' | -50.8 | NC_005882.1 | + | 20382 | 0.68 | 0.757082 |
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Target: 5'- uGGAUCGCUUCggUcGCUGUCUggaCGGc- -3' miRNA: 3'- -CCUAGCGAAGgaA-CGGCAGAa--GCUac -5' |
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| 27597 | 5' | -50.8 | NC_005882.1 | + | 20199 | 0.68 | 0.735078 |
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Target: 5'- cGGAaCGaUUCCUUGCCGaaCUUCGGa- -3' miRNA: 3'- -CCUaGCgAAGGAACGGCa-GAAGCUac -5' |
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| 27597 | 5' | -50.8 | NC_005882.1 | + | 15615 | 0.68 | 0.723897 |
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Target: 5'- cGGGagGCcgUCgaucgUGCCGUCUUCGAa- -3' miRNA: 3'- -CCUagCGaaGGa----ACGGCAGAAGCUac -5' |
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| 27597 | 5' | -50.8 | NC_005882.1 | + | 17817 | 0.69 | 0.69667 |
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Target: 5'- cGGAUCGUcguaacggaacaCCUUGCCGuUCUUgccCGAUGg -3' miRNA: 3'- -CCUAGCGaa----------GGAACGGC-AGAA---GCUAC- -5' |
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| 27597 | 5' | -50.8 | NC_005882.1 | + | 14121 | 0.7 | 0.643515 |
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Target: 5'- cGGUCGCUUCC-UGCagGUUgaCGAUGg -3' miRNA: 3'- cCUAGCGAAGGaACGg-CAGaaGCUAC- -5' |
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| 27597 | 5' | -50.8 | NC_005882.1 | + | 20883 | 0.72 | 0.528515 |
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Target: 5'- --uUCGag--CUUGCCGUCUUCGAUGc -3' miRNA: 3'- ccuAGCgaagGAACGGCAGAAGCUAC- -5' |
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| 27597 | 5' | -50.8 | NC_005882.1 | + | 20532 | 1.12 | 0.001115 |
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Target: 5'- cGGAUCGCUUCCUUGCCGUCUUCGAUGa -3' miRNA: 3'- -CCUAGCGAAGGAACGGCAGAAGCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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