Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27599 | 5' | -52 | NC_005882.1 | + | 21604 | 1.1 | 0.001247 |
Target: 5'- uGACCGUGACAUCCUUGAUGACCGUCGg -3' miRNA: 3'- -CUGGCACUGUAGGAACUACUGGCAGC- -5' |
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27599 | 5' | -52 | NC_005882.1 | + | 15632 | 0.7 | 0.567553 |
Target: 5'- aGCaCGUcGGCAUCCUucgGGaGGCCGUCGa -3' miRNA: 3'- cUG-GCA-CUGUAGGAa--CUaCUGGCAGC- -5' |
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27599 | 5' | -52 | NC_005882.1 | + | 30752 | 0.67 | 0.71612 |
Target: 5'- uGCCGccGGCAUCCaUGAUGguGCCaGUCGc -3' miRNA: 3'- cUGGCa-CUGUAGGaACUAC--UGG-CAGC- -5' |
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27599 | 5' | -52 | NC_005882.1 | + | 423 | 0.67 | 0.738297 |
Target: 5'- cGCCG-GACcgCCUUGAUGAUC-UUGu -3' miRNA: 3'- cUGGCaCUGuaGGAACUACUGGcAGC- -5' |
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27599 | 5' | -52 | NC_005882.1 | + | 4010 | 0.66 | 0.801599 |
Target: 5'- cGGCCaUGAUugcuUCCgcGAUGugCGUCGc -3' miRNA: 3'- -CUGGcACUGu---AGGaaCUACugGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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