miRNA: 3'- -UCUGa--CCUCGG-GAUGUC-UCgGGCGgg -5'
miRNA: 3'- -UCUGACC------UCGGGaugUCUCG-GGCGGG- -5'
miRNA: 3'- uCUGACC-UCGGGau---GUCucGGGCG-GG- -5'
miRNA: 3'- -UCUGac-CUCGgGAUGuCUCGG-GCGGg -5'
miRNA: 3'- -UCUGaCCUCGGGA-UGucUCGGGcGGG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2760 | 3' | -60.2 | NC_001491.2 | + | 147746 | 0.67 | 0.680515 |
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Target: 5'- cGGGCcggGGGGCCgCUGCAGcGGgCCGCg- -3' miRNA: 3'- -UCUGa--CCUCGG-GAUGUC-UCgGGCGgg -5' |
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| 2760 | 3' | -60.2 | NC_001491.2 | + | 74411 | 0.66 | 0.690401 |
|
Target: 5'- uAGACUGGuugauuGGCCC---AGAGCuCCGUUCg -3' miRNA: 3'- -UCUGACC------UCGGGaugUCUCG-GGCGGG- -5' |
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| 2760 | 3' | -60.2 | NC_001491.2 | + | 114406 | 0.66 | 0.710027 |
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Target: 5'- cGACUGGgAGCCCggguuCGGcuCUCGCuCCa -3' miRNA: 3'- uCUGACC-UCGGGau---GUCucGGGCG-GG- -5' |
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| 2760 | 3' | -60.2 | NC_001491.2 | + | 139782 | 0.66 | 0.719749 |
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Target: 5'- cGGACgcaGAGCgCUACuGGGCCgCGCUa -3' miRNA: 3'- -UCUGac-CUCGgGAUGuCUCGG-GCGGg -5' |
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| 2760 | 3' | -60.2 | NC_001491.2 | + | 117644 | 0.66 | 0.7294 |
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Target: 5'- cGGGCcGGAGCCCUcACc-GGCUC-CCCa -3' miRNA: 3'- -UCUGaCCUCGGGA-UGucUCGGGcGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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