Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2760 | 3' | -60.2 | NC_001491.2 | + | 45132 | 0.7 | 0.493822 |
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Target: 5'- uGGCcGGGGCCgCgGCGGGGgCCGCCg -3' miRNA: 3'- uCUGaCCUCGG-GaUGUCUCgGGCGGg -5' |
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| 2760 | 3' | -60.2 | NC_001491.2 | + | 148043 | 0.7 | 0.484498 |
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Target: 5'- gAGACguccGGGGCCCcggucCAGuuGCCgGCCCa -3' miRNA: 3'- -UCUGa---CCUCGGGau---GUCu-CGGgCGGG- -5' |
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| 2760 | 3' | -60.2 | NC_001491.2 | + | 116564 | 0.71 | 0.430457 |
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Target: 5'- cGGACUGGAcCCCaGCgAGAuccccucucccGCCUGCCCu -3' miRNA: 3'- -UCUGACCUcGGGaUG-UCU-----------CGGGCGGG- -5' |
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| 2760 | 3' | -60.2 | NC_001491.2 | + | 114482 | 0.74 | 0.285304 |
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Target: 5'- -uGCUGGAGCCCgacgccgagGCcGAGCCCcuggugcuGCCCa -3' miRNA: 3'- ucUGACCUCGGGa--------UGuCUCGGG--------CGGG- -5' |
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| 2760 | 3' | -60.2 | NC_001491.2 | + | 147906 | 0.75 | 0.248258 |
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Target: 5'- gGGGCgGGAGgCCg--GGAGUCCGCCCa -3' miRNA: 3'- -UCUGaCCUCgGGaugUCUCGGGCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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