miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27609 5' -56.1 NC_005882.1 + 25077 0.65 0.558491
Target:  5'- cGAugGCCuUGAUCgCCgccgcgaugagguaGGAGCCGGCcACg -3'
miRNA:   3'- aCU--UGGcACUAG-GG--------------UCUCGGCCGaUG- -5'
27609 5' -56.1 NC_005882.1 + 19400 0.66 0.51129
Target:  5'- cUGAucaGCCGUG-UCCCGGccgaauugcgucagcAGCCauucgcgcaGGCUGCg -3'
miRNA:   3'- -ACU---UGGCACuAGGGUC---------------UCGG---------CCGAUG- -5'
27609 5' -56.1 NC_005882.1 + 26675 0.67 0.485669
Target:  5'- -cAACCGUGAUCCCGaGGuCCGcaauCUGCg -3'
miRNA:   3'- acUUGGCACUAGGGUcUC-GGCc---GAUG- -5'
27609 5' -56.1 NC_005882.1 + 25044 0.67 0.475174
Target:  5'- cGGcCCG-GcgCCC-GAGCCGGCcGCa -3'
miRNA:   3'- aCUuGGCaCuaGGGuCUCGGCCGaUG- -5'
27609 5' -56.1 NC_005882.1 + 12292 0.72 0.251034
Target:  5'- cGGccGCCGUGAugcgcUCCCAGAgguGCCGGUcgUACc -3'
miRNA:   3'- aCU--UGGCACU-----AGGGUCU---CGGCCG--AUG- -5'
27609 5' -56.1 NC_005882.1 + 27738 1.09 0.000472
Target:  5'- gUGAACCGUGAUCCCAGAGCCGGCUACc -3'
miRNA:   3'- -ACUUGGCACUAGGGUCUCGGCCGAUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.