Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27610 | 5' | -56 | NC_005882.1 | + | 31925 | 0.66 | 0.562398 |
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Target: 5'- aCGCUGCCUgGCUGAacgccGUUGacggaaaccagccgGCCgUCGCGc -3' miRNA: 3'- -GUGACGGA-CGACU-----UAGCa-------------CGG-AGCGC- -5' |
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| 27610 | 5' | -56 | NC_005882.1 | + | 1367 | 0.67 | 0.492458 |
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Target: 5'- aGCUGUUUGCUGcg-CGcuuCCUCGCGg -3' miRNA: 3'- gUGACGGACGACuuaGCac-GGAGCGC- -5' |
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| 27610 | 5' | -56 | NC_005882.1 | + | 23269 | 0.67 | 0.461056 |
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Target: 5'- aGC-GCCUGCgcGAgcGUCGUGCCgaucggCGCa -3' miRNA: 3'- gUGaCGGACGa-CU--UAGCACGGa-----GCGc -5' |
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| 27610 | 5' | -56 | NC_005882.1 | + | 11635 | 0.68 | 0.427778 |
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Target: 5'- aACUGCCcGUcgcccucuacaaacUGAGucUCGUcaGCCUCGCGg -3' miRNA: 3'- gUGACGGaCG--------------ACUU--AGCA--CGGAGCGC- -5' |
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| 27610 | 5' | -56 | NC_005882.1 | + | 14500 | 0.69 | 0.373804 |
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Target: 5'- gGCUGCgagcaacGCUGAAUCGgcUGCUUCGCc -3' miRNA: 3'- gUGACGga-----CGACUUAGC--ACGGAGCGc -5' |
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| 27610 | 5' | -56 | NC_005882.1 | + | 18427 | 0.7 | 0.306211 |
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Target: 5'- cCGCUGuCCUGCaGGAUCaucagaccgGCCUUGCGa -3' miRNA: 3'- -GUGAC-GGACGaCUUAGca-------CGGAGCGC- -5' |
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| 27610 | 5' | -56 | NC_005882.1 | + | 8037 | 0.73 | 0.21118 |
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Target: 5'- cCAUUGCUUGCUGGAUCGUcGCgaUCGUu -3' miRNA: 3'- -GUGACGGACGACUUAGCA-CGg-AGCGc -5' |
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| 27610 | 5' | -56 | NC_005882.1 | + | 28381 | 1.07 | 0.000592 |
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Target: 5'- uCACUGCCUGCUGAAUCGUGCCUCGCGc -3' miRNA: 3'- -GUGACGGACGACUUAGCACGGAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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