miRNA display CGI


Results 21 - 23 of 23 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27612 3' -60.8 NC_005882.1 + 21665 0.77 0.055492
Target:  5'- aCGG-UGUCCGGGAGCGCgGCG-CCUu -3'
miRNA:   3'- gGCCgACAGGUCCUCGUGgCGCuGGA- -5'
27612 3' -60.8 NC_005882.1 + 6470 0.78 0.042809
Target:  5'- gUGGCUGUcgcCCAGGcAGCGCgCGCGGCCg -3'
miRNA:   3'- gGCCGACA---GGUCC-UCGUG-GCGCUGGa -5'
27612 3' -60.8 NC_005882.1 + 30380 1.1 0.000164
Target:  5'- aCCGGCUGUCCAGGAGCACCGCGACCUg -3'
miRNA:   3'- -GGCCGACAGGUCCUCGUGGCGCUGGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.