Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
27614 | 3' | -53.6 | NC_005882.1 | + | 33412 | 0.76 | 0.230434 |
Target: 5'- ---cCGCuGUUCaACGUUGCCGUCGugGc -3' miRNA: 3'- gucaGCG-CAAG-UGCAACGGCAGCugC- -5' |
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27614 | 3' | -53.6 | NC_005882.1 | + | 28362 | 0.8 | 0.11431 |
Target: 5'- gCAGUcCGCGaugCGCGUgGCCGUCGACGc -3' miRNA: 3'- -GUCA-GCGCaa-GUGCAaCGGCAGCUGC- -5' |
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27614 | 3' | -53.6 | NC_005882.1 | + | 30729 | 1.08 | 0.001165 |
Target: 5'- cCAGUCGCGUUCACGUUGCCGUCGACGu -3' miRNA: 3'- -GUCAGCGCAAGUGCAACGGCAGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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