Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27621 | 5' | -59.8 | NC_005882.1 | + | 18709 | 0.66 | 0.341216 |
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Target: 5'- gGGUGGUcCGGagGCGCGCAGagcGCGAu- -3' miRNA: 3'- aCCGCCA-GUCg-UGCGCGUCa--CGCUca -5' |
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| 27621 | 5' | -59.8 | NC_005882.1 | + | 26759 | 0.66 | 0.33299 |
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Target: 5'- gGGCGG-CcGCGaacUGCGCGGUGCGcuGGa -3' miRNA: 3'- aCCGCCaGuCGU---GCGCGUCACGC--UCa -5' |
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| 27621 | 5' | -59.8 | NC_005882.1 | + | 10828 | 0.66 | 0.33299 |
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Target: 5'- aGGCGGUC-GCGCuCGUcGcUGCGGGg -3' miRNA: 3'- aCCGCCAGuCGUGcGCGuC-ACGCUCa -5' |
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| 27621 | 5' | -59.8 | NC_005882.1 | + | 27631 | 0.66 | 0.32491 |
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Target: 5'- cGGCGG-CcGCGCGCGCg--GCGAc- -3' miRNA: 3'- aCCGCCaGuCGUGCGCGucaCGCUca -5' |
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| 27621 | 5' | -59.8 | NC_005882.1 | + | 22107 | 0.68 | 0.272452 |
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Target: 5'- cGGCGGUCAGCgACaaGUuucucgGCGAGUc -3' miRNA: 3'- aCCGCCAGUCG-UGcgCGuca---CGCUCA- -5' |
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| 27621 | 5' | -59.8 | NC_005882.1 | + | 11511 | 0.7 | 0.198623 |
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Target: 5'- -aGCGGUCGGCuuGCGCGGcguuccUGCGAu- -3' miRNA: 3'- acCGCCAGUCGugCGCGUC------ACGCUca -5' |
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| 27621 | 5' | -59.8 | NC_005882.1 | + | 25561 | 0.71 | 0.146889 |
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Target: 5'- aGGCGGUCguacuGGC-CGCGCAGcucgGCGAu- -3' miRNA: 3'- aCCGCCAG-----UCGuGCGCGUCa---CGCUca -5' |
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| 27621 | 5' | -59.8 | NC_005882.1 | + | 3474 | 0.71 | 0.146889 |
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Target: 5'- cGGCGGUgacgacCGGCACGCcCAGgcccgcgGCGAGa -3' miRNA: 3'- aCCGCCA------GUCGUGCGcGUCa------CGCUCa -5' |
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| 27621 | 5' | -59.8 | NC_005882.1 | + | 27569 | 0.73 | 0.104696 |
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Target: 5'- cGGCGGUCGccGCGCGCGCGGccgccggcaugGCGAc- -3' miRNA: 3'- aCCGCCAGU--CGUGCGCGUCa----------CGCUca -5' |
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| 27621 | 5' | -59.8 | NC_005882.1 | + | 32270 | 1.06 | 0.000298 |
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Target: 5'- cUGGCGGUCAGCACGCGCAGUGCGAGUc -3' miRNA: 3'- -ACCGCCAGUCGUGCGCGUCACGCUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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