Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27623 | 3' | -52.1 | NC_005882.1 | + | 32841 | 1.08 | 0.001648 |
Target: 5'- cACACGACGGUAUUCGAGACUCGCGAGu -3' miRNA: 3'- -UGUGCUGCCAUAAGCUCUGAGCGCUC- -5' |
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27623 | 3' | -52.1 | NC_005882.1 | + | 31748 | 0.69 | 0.636548 |
Target: 5'- aGCACGGCGGgc-UCccGGCUCGCGu- -3' miRNA: 3'- -UGUGCUGCCauaAGcuCUGAGCGCuc -5' |
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27623 | 3' | -52.1 | NC_005882.1 | + | 4522 | 0.67 | 0.716009 |
Target: 5'- cGCAgGucgaGGUcgUCGAGGCUgCGCGGc -3' miRNA: 3'- -UGUgCug--CCAuaAGCUCUGA-GCGCUc -5' |
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27623 | 3' | -52.1 | NC_005882.1 | + | 26284 | 0.67 | 0.759713 |
Target: 5'- cGC-CGGCGGcUAUUCGAGcuacgaagGCUCGCu-- -3' miRNA: 3'- -UGuGCUGCC-AUAAGCUC--------UGAGCGcuc -5' |
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27623 | 3' | -52.1 | NC_005882.1 | + | 26190 | 0.66 | 0.791049 |
Target: 5'- uCGCGGCcGacUUCGAGGCUgGCGAu -3' miRNA: 3'- uGUGCUGcCauAAGCUCUGAgCGCUc -5' |
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27623 | 3' | -52.1 | NC_005882.1 | + | 8280 | 0.66 | 0.820769 |
Target: 5'- gACGCGuGCGGUGccgauUUCGucACUCGCuGGGu -3' miRNA: 3'- -UGUGC-UGCCAU-----AAGCucUGAGCG-CUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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