Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27625 | 3' | -56.8 | NC_005882.1 | + | 9231 | 0.66 | 0.494365 |
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Target: 5'- cGAG-CGCCUGcgcgaggagcguGAGCGGUUGgGGUAUa -3' miRNA: 3'- -CUCgGCGGAC------------CUUGCCAACaCCGUAg -5' |
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| 27625 | 3' | -56.8 | NC_005882.1 | + | 7229 | 0.67 | 0.412982 |
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Target: 5'- aAGCgUGCCUGGAGguUGGUcGUGGCcuUCg -3' miRNA: 3'- cUCG-GCGGACCUU--GCCAaCACCGu-AG- -5' |
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| 27625 | 3' | -56.8 | NC_005882.1 | + | 1333 | 0.68 | 0.356774 |
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Target: 5'- cAGaaaCGCCUGGAGCGGgccGUGccgcucgaugucuGCAUCa -3' miRNA: 3'- cUCg--GCGGACCUUGCCaa-CAC-------------CGUAG- -5' |
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| 27625 | 3' | -56.8 | NC_005882.1 | + | 15690 | 0.68 | 0.348949 |
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Target: 5'- -cGCCGCCgu-AGCGGUUGcucUGGCGUa -3' miRNA: 3'- cuCGGCGGaccUUGCCAAC---ACCGUAg -5' |
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| 27625 | 3' | -56.8 | NC_005882.1 | + | 7138 | 0.69 | 0.307729 |
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Target: 5'- uGAGCCGCgUGuagcucAACGGUUcaUGGCGUCg -3' miRNA: 3'- -CUCGGCGgACc-----UUGCCAAc-ACCGUAG- -5' |
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| 27625 | 3' | -56.8 | NC_005882.1 | + | 33865 | 1.08 | 0.000388 |
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Target: 5'- aGAGCCGCCUGGAACGGUUGUGGCAUCc -3' miRNA: 3'- -CUCGGCGGACCUUGCCAACACCGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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