Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27629 | 5' | -50.9 | NC_005882.1 | + | 4927 | 0.66 | 0.82891 |
Target: 5'- cGUCGAAc-UCgcGCCGcUCUCGCUGa- -3' miRNA: 3'- -CAGCUUuuAGa-CGGCuAGAGCGACcc -5' |
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27629 | 5' | -50.9 | NC_005882.1 | + | 28572 | 0.67 | 0.767876 |
Target: 5'- cUCGGGAAUCUgGUCGGUCagggacgccagCGCUGGc -3' miRNA: 3'- cAGCUUUUAGA-CGGCUAGa----------GCGACCc -5' |
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27629 | 5' | -50.9 | NC_005882.1 | + | 4873 | 0.67 | 0.778515 |
Target: 5'- uGUCGAAg--CUGCCGGcgUCGaCUGGa -3' miRNA: 3'- -CAGCUUuuaGACGGCUagAGC-GACCc -5' |
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27629 | 5' | -50.9 | NC_005882.1 | + | 29272 | 0.68 | 0.712613 |
Target: 5'- cGUCGAAgccAAUCUcGUCGAUCUCGaaGcGGc -3' miRNA: 3'- -CAGCUU---UUAGA-CGGCUAGAGCgaC-CC- -5' |
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27629 | 5' | -50.9 | NC_005882.1 | + | 8286 | 0.71 | 0.572814 |
Target: 5'- uUCGAGGAcgCgugcggUGCCGAUuucgucaCUCGCUGGGu -3' miRNA: 3'- cAGCUUUUa-G------ACGGCUA-------GAGCGACCC- -5' |
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27629 | 5' | -50.9 | NC_005882.1 | + | 511 | 1.12 | 0.001115 |
Target: 5'- cGUCGAAAAUCUGCCGAUCUCGCUGGGa -3' miRNA: 3'- -CAGCUUUUAGACGGCUAGAGCGACCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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