Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27631 | 3' | -52.8 | NC_005882.1 | + | 16970 | 0.66 | 0.775386 |
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Target: 5'- gCCGGCGUgAAACGCGcgccggCUUGCGu--- -3' miRNA: 3'- -GGCCGCAaUUUGCGUa-----GGACGCcucu -5' |
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| 27631 | 3' | -52.8 | NC_005882.1 | + | 10828 | 0.66 | 0.754222 |
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Target: 5'- -aGGCGguc-GCGCucGUCgCUGCGGGGc -3' miRNA: 3'- ggCCGCaauuUGCG--UAG-GACGCCUCu -5' |
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| 27631 | 3' | -52.8 | NC_005882.1 | + | 23025 | 0.66 | 0.743432 |
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Target: 5'- aUCGGCGccgUGAACGCAgCCggaaugcgcaCGGGGAu -3' miRNA: 3'- -GGCCGCa--AUUUGCGUaGGac--------GCCUCU- -5' |
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| 27631 | 3' | -52.8 | NC_005882.1 | + | 18822 | 0.67 | 0.7215 |
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Target: 5'- gCCGGCGUgauACGCGcgaUGCaGGGAg -3' miRNA: 3'- -GGCCGCAauuUGCGUaggACGcCUCU- -5' |
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| 27631 | 3' | -52.8 | NC_005882.1 | + | 31240 | 0.67 | 0.676589 |
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Target: 5'- uCCGGCGcaggucGAACGCGUCCUG-GucGAc -3' miRNA: 3'- -GGCCGCaa----UUUGCGUAGGACgCcuCU- -5' |
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| 27631 | 3' | -52.8 | NC_005882.1 | + | 35522 | 0.67 | 0.676589 |
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Target: 5'- cCCGGUGUgaaucuUGGGCGC-UCC-GCGGcGAa -3' miRNA: 3'- -GGCCGCA------AUUUGCGuAGGaCGCCuCU- -5' |
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| 27631 | 3' | -52.8 | NC_005882.1 | + | 20440 | 0.73 | 0.367544 |
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Target: 5'- cCCGGCGUagcUGuuCGgGUUCUGCGcGAGGu -3' miRNA: 3'- -GGCCGCA---AUuuGCgUAGGACGC-CUCU- -5' |
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| 27631 | 3' | -52.8 | NC_005882.1 | + | 17105 | 0.78 | 0.179526 |
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Target: 5'- gCGGCGUcGAGCGCGUCCUGguCGGGc- -3' miRNA: 3'- gGCCGCAaUUUGCGUAGGAC--GCCUcu -5' |
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| 27631 | 3' | -52.8 | NC_005882.1 | + | 21300 | 0.82 | 0.094455 |
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Target: 5'- gCCGGCGacgucaucCGCAUCCUGCGGGGu -3' miRNA: 3'- -GGCCGCaauuu---GCGUAGGACGCCUCu -5' |
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| 27631 | 3' | -52.8 | NC_005882.1 | + | 1261 | 1.11 | 0.000805 |
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Target: 5'- gCCGGCGUUAAACGCAUCCUGCGGAGAa -3' miRNA: 3'- -GGCCGCAAUUUGCGUAGGACGCCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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