Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27633 | 5' | -56.2 | NC_005882.1 | + | 30201 | 0.67 | 0.531322 |
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Target: 5'- cCGGCUUCGCGcGcGUACGGcugaCCGAccUCg -3' miRNA: 3'- -GCUGAAGCGCuC-CAUGCCc---GGCU--AGa -5' |
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| 27633 | 5' | -56.2 | NC_005882.1 | + | 22654 | 0.67 | 0.499388 |
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Target: 5'- uGACcggCcCGAucGGUGCGGuGCCGAUCa -3' miRNA: 3'- gCUGaa-GcGCU--CCAUGCC-CGGCUAGa -5' |
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| 27633 | 5' | -56.2 | NC_005882.1 | + | 23233 | 0.69 | 0.409405 |
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Target: 5'- uGGCcgggCGCGGGccgGCGGGCaCGAUCa -3' miRNA: 3'- gCUGaa--GCGCUCca-UGCCCG-GCUAGa -5' |
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| 27633 | 5' | -56.2 | NC_005882.1 | + | 5240 | 0.69 | 0.400038 |
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Target: 5'- uGACUg-GCGAGG-ACGGGCgCGAg-- -3' miRNA: 3'- gCUGAagCGCUCCaUGCCCG-GCUaga -5' |
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| 27633 | 5' | -56.2 | NC_005882.1 | + | 2934 | 0.7 | 0.363975 |
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Target: 5'- gCGGC-UCGC-AGGU-CGGuGCCGGUCUu -3' miRNA: 3'- -GCUGaAGCGcUCCAuGCC-CGGCUAGA- -5' |
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| 27633 | 5' | -56.2 | NC_005882.1 | + | 3409 | 0.72 | 0.27685 |
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Target: 5'- aGACUUCGC--GGUGCGGGCaGGUUc -3' miRNA: 3'- gCUGAAGCGcuCCAUGCCCGgCUAGa -5' |
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| 27633 | 5' | -56.2 | NC_005882.1 | + | 4947 | 0.72 | 0.249566 |
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Target: 5'- uCGGCUUUGuCGAGGUgcuuguACGGGUCGAg-- -3' miRNA: 3'- -GCUGAAGC-GCUCCA------UGCCCGGCUaga -5' |
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| 27633 | 5' | -56.2 | NC_005882.1 | + | 3271 | 0.73 | 0.218627 |
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Target: 5'- cCGACgugcugCGCGAGcGUGCGGGCgGcGUCa -3' miRNA: 3'- -GCUGaa----GCGCUC-CAUGCCCGgC-UAGa -5' |
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| 27633 | 5' | -56.2 | NC_005882.1 | + | 15497 | 0.74 | 0.201679 |
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Target: 5'- gCGACUUCuGCGAGGUAUuGGCCG-UUg -3' miRNA: 3'- -GCUGAAG-CGCUCCAUGcCCGGCuAGa -5' |
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| 27633 | 5' | -56.2 | NC_005882.1 | + | 3236 | 1.09 | 0.000572 |
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Target: 5'- aCGACUUCGCGAGGUACGGGCCGAUCUg -3' miRNA: 3'- -GCUGAAGCGCUCCAUGCCCGGCUAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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