Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27634 | 5' | -55.3 | NC_005882.1 | + | 22867 | 0.69 | 0.417305 |
Target: 5'- uGCGCGAGuuuUGCACggcccguugCCGCGUCa---- -3' miRNA: 3'- -UGCGCUCu--GCGUGa--------GGCGCAGcuuuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 30249 | 0.7 | 0.370408 |
Target: 5'- cGCGCGAagccgggcuucGACGCACUgaCCGUGcCGAAc- -3' miRNA: 3'- -UGCGCU-----------CUGCGUGA--GGCGCaGCUUuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 17940 | 0.7 | 0.352683 |
Target: 5'- uGCGCGAGGuCGCGacgUUCCGCacGUCGAc-- -3' miRNA: 3'- -UGCGCUCU-GCGU---GAGGCG--CAGCUuuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 8405 | 0.74 | 0.197635 |
Target: 5'- cGCGCGAGcgGCGCAauCUCaGCGUCGAAc- -3' miRNA: 3'- -UGCGCUC--UGCGU--GAGgCGCAGCUUuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 5225 | 0.75 | 0.181821 |
Target: 5'- gGCGCGAGACGUACUUCGuCGUUc---- -3' miRNA: 3'- -UGCGCUCUGCGUGAGGC-GCAGcuuuu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 17274 | 0.75 | 0.173852 |
Target: 5'- cGCGCGGGcaagccguuccugcaGCGCG-UCCGCGUCGAGGc -3' miRNA: 3'- -UGCGCUC---------------UGCGUgAGGCGCAGCUUUu -5' |
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27634 | 5' | -55.3 | NC_005882.1 | + | 3362 | 1.05 | 0.001011 |
Target: 5'- gACGCGAGACGCACUCCGCGUCGAAAAc -3' miRNA: 3'- -UGCGCUCUGCGUGAGGCGCAGCUUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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