Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27635 | 5' | -58.8 | NC_005882.1 | + | 27285 | 0.65 | 0.430338 |
Target: 5'- cGGGGGcaUCUGggcuucgauucguACCGCGuucGCGGGUGu -3' miRNA: 3'- uCCUCCaaAGGC-------------UGGCGCu--CGCCCAU- -5' |
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27635 | 5' | -58.8 | NC_005882.1 | + | 13647 | 0.68 | 0.32491 |
Target: 5'- aAGGAaccgUCCGGCCGCuGGUGGGg- -3' miRNA: 3'- -UCCUccaaAGGCUGGCGcUCGCCCau -5' |
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27635 | 5' | -58.8 | NC_005882.1 | + | 27286 | 0.68 | 0.294058 |
Target: 5'- -uGAGGUacgcgCCGACCGCGAuCGcGGUGa -3' miRNA: 3'- ucCUCCAaa---GGCUGGCGCUcGC-CCAU- -5' |
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27635 | 5' | -58.8 | NC_005882.1 | + | 4772 | 0.69 | 0.245651 |
Target: 5'- -cGAGcUUaCCGGCcCGCGAGCGGGUc -3' miRNA: 3'- ucCUCcAAaGGCUG-GCGCUCGCCCAu -5' |
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27635 | 5' | -58.8 | NC_005882.1 | + | 3680 | 0.72 | 0.154851 |
Target: 5'- cGGAGGUauucgUCCaggcgcgGGCCGuCGAGCGGGg- -3' miRNA: 3'- uCCUCCAa----AGG-------CUGGC-GCUCGCCCau -5' |
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27635 | 5' | -58.8 | NC_005882.1 | + | 3526 | 1.06 | 0.000428 |
Target: 5'- cAGGAGGUUUCCGACCGCGAGCGGGUAc -3' miRNA: 3'- -UCCUCCAAAGGCUGGCGCUCGCCCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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