Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27636 | 5' | -56.4 | NC_005882.1 | + | 21740 | 0.66 | 0.501999 |
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Target: 5'- cUCG-GGUCGCcgauCGUCAGCuuGUUGUCg -3' miRNA: 3'- aAGCuCCAGCGc---GUAGUCG--CAGCAGa -5' |
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| 27636 | 5' | -56.4 | NC_005882.1 | + | 26180 | 0.67 | 0.470409 |
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Target: 5'- cUUCGAGGcUgGCgaugaucuGCGUCAGCG-CGUCc -3' miRNA: 3'- -AAGCUCC-AgCG--------CGUAGUCGCaGCAGa -5' |
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| 27636 | 5' | -56.4 | NC_005882.1 | + | 31039 | 0.67 | 0.460105 |
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Target: 5'- -gCGAGGUCGUGCGugUCAuucCGUCGaUCg -3' miRNA: 3'- aaGCUCCAGCGCGU--AGUc--GCAGC-AGa -5' |
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| 27636 | 5' | -56.4 | NC_005882.1 | + | 17136 | 0.67 | 0.460105 |
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Target: 5'- aUUC-AGGUCGCGCGcggacaagguguUCAGUGcggCGUCg -3' miRNA: 3'- -AAGcUCCAGCGCGU------------AGUCGCa--GCAGa -5' |
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| 27636 | 5' | -56.4 | NC_005882.1 | + | 12074 | 0.67 | 0.439863 |
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Target: 5'- -gCGcGGaCGC-CGUCAGCGUCGUUg -3' miRNA: 3'- aaGCuCCaGCGcGUAGUCGCAGCAGa -5' |
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| 27636 | 5' | -56.4 | NC_005882.1 | + | 16159 | 0.68 | 0.410479 |
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Target: 5'- gUCGAGGUcCGCGaagaCGUCGGCGaUCGaCUu -3' miRNA: 3'- aAGCUCCA-GCGC----GUAGUCGC-AGCaGA- -5' |
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| 27636 | 5' | -56.4 | NC_005882.1 | + | 7695 | 0.68 | 0.400958 |
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Target: 5'- gUCGAGGcgcUCGCGgG-CGGCGUCGa-- -3' miRNA: 3'- aAGCUCC---AGCGCgUaGUCGCAGCaga -5' |
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| 27636 | 5' | -56.4 | NC_005882.1 | + | 2159 | 0.7 | 0.321872 |
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Target: 5'- uUUCGGGG-CGUGCGUC--CGUCGUCc -3' miRNA: 3'- -AAGCUCCaGCGCGUAGucGCAGCAGa -5' |
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| 27636 | 5' | -56.4 | NC_005882.1 | + | 23648 | 0.71 | 0.268838 |
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Target: 5'- cUCGAaGUUGCGCAUguGCGUCacgGUCg -3' miRNA: 3'- aAGCUcCAGCGCGUAguCGCAG---CAGa -5' |
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| 27636 | 5' | -56.4 | NC_005882.1 | + | 4133 | 1.04 | 0.000902 |
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Target: 5'- cUUCGAGGUCGCGCAUCAGCGUCGUCUu -3' miRNA: 3'- -AAGCUCCAGCGCGUAGUCGCAGCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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