Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27637 | 3' | -59.1 | NC_005882.1 | + | 33097 | 0.66 | 0.426419 |
Target: 5'- uGCGGGUCGGGcG-CGgCGAuauGCUGCGc -3' miRNA: 3'- gCGUCCAGCUC-CaGCaGCUc--CGACGC- -5' |
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27637 | 3' | -59.1 | NC_005882.1 | + | 32421 | 0.66 | 0.416985 |
Target: 5'- aGCGGcgacauGUCGAuGUUGUCGAGGC-GCu -3' miRNA: 3'- gCGUC------CAGCUcCAGCAGCUCCGaCGc -5' |
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27637 | 3' | -59.1 | NC_005882.1 | + | 22465 | 0.66 | 0.398504 |
Target: 5'- gCGCGGG-CuuGGUCGaCGGcGCUGCGg -3' miRNA: 3'- -GCGUCCaGcuCCAGCaGCUcCGACGC- -5' |
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27637 | 3' | -59.1 | NC_005882.1 | + | 7679 | 0.67 | 0.351311 |
Target: 5'- gGCGGGuuugaaugcugUCGAGGcgcucgcgggcggCGUCGAGGCUaCGg -3' miRNA: 3'- gCGUCC-----------AGCUCCa------------GCAGCUCCGAcGC- -5' |
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27637 | 3' | -59.1 | NC_005882.1 | + | 33003 | 0.71 | 0.214464 |
Target: 5'- gCGCAGGUCGAGca-GUCGcagaccGGGCUuGCGc -3' miRNA: 3'- -GCGUCCAGCUCcagCAGC------UCCGA-CGC- -5' |
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27637 | 3' | -59.1 | NC_005882.1 | + | 4521 | 1.08 | 0.000344 |
Target: 5'- cCGCAGGUCGAGGUCGUCGAGGCUGCGc -3' miRNA: 3'- -GCGUCCAGCUCCAGCAGCUCCGACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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