Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27639 | 3' | -53.9 | NC_005882.1 | + | 27570 | 0.66 | 0.678439 |
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Target: 5'- gGCggUCGCCGcgCGC-GCGGCcGcCg -3' miRNA: 3'- -CGaaAGCGGCuaGUGaCGCCGuCaGa -5' |
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| 27639 | 3' | -53.9 | NC_005882.1 | + | 36554 | 0.66 | 0.667031 |
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Target: 5'- cGCUUUCGCCGcgauCUG-GGUAGUg- -3' miRNA: 3'- -CGAAAGCGGCuaguGACgCCGUCAga -5' |
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| 27639 | 3' | -53.9 | NC_005882.1 | + | 35077 | 0.67 | 0.575459 |
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Target: 5'- cGCUccCuaUGAUCGaUGCGGCGGUCg -3' miRNA: 3'- -CGAaaGcgGCUAGUgACGCCGUCAGa -5' |
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| 27639 | 3' | -53.9 | NC_005882.1 | + | 8355 | 0.67 | 0.574324 |
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Target: 5'- ---cUCGCCGAUCACUGgacucucuacaaaUGGUuccAGUCa -3' miRNA: 3'- cgaaAGCGGCUAGUGAC-------------GCCG---UCAGa -5' |
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| 27639 | 3' | -53.9 | NC_005882.1 | + | 29941 | 0.68 | 0.564135 |
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Target: 5'- cGCgacaUCGUcuCGAUCGCgGCGGCGG-CUa -3' miRNA: 3'- -CGaa--AGCG--GCUAGUGaCGCCGUCaGA- -5' |
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| 27639 | 3' | -53.9 | NC_005882.1 | + | 4913 | 0.68 | 0.552871 |
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Target: 5'- cGCUcUCGCUGAUCGagGCGGCAa--- -3' miRNA: 3'- -CGAaAGCGGCUAGUgaCGCCGUcaga -5' |
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| 27639 | 3' | -53.9 | NC_005882.1 | + | 19430 | 0.7 | 0.425061 |
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Target: 5'- cGCaUUCGCCGAaCGCgggGCGGCAa--- -3' miRNA: 3'- -CGaAAGCGGCUaGUGa--CGCCGUcaga -5' |
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| 27639 | 3' | -53.9 | NC_005882.1 | + | 21191 | 0.71 | 0.386478 |
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Target: 5'- cGCUUgcUCGCCGA-CAU--CGGCGGUCg -3' miRNA: 3'- -CGAA--AGCGGCUaGUGacGCCGUCAGa -5' |
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| 27639 | 3' | -53.9 | NC_005882.1 | + | 12517 | 0.72 | 0.341611 |
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Target: 5'- uGCUUUCGCgCGGccagugugUCGCgGCGGCGGcCg -3' miRNA: 3'- -CGAAAGCG-GCU--------AGUGaCGCCGUCaGa -5' |
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| 27639 | 3' | -53.9 | NC_005882.1 | + | 5106 | 1.1 | 0.000718 |
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Target: 5'- aGCUUUCGCCGAUCACUGCGGCAGUCUc -3' miRNA: 3'- -CGAAAGCGGCUAGUGACGCCGUCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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