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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27641 | 3' | -57.1 | NC_005882.1 | + | 25758 | 0.66 | 0.489463 |
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Target: 5'- uGUCGCGCag-GCCGGCUucgaagGUGgCGg-- -3' miRNA: 3'- gCAGCGUGguaCGGCCGA------CAC-GCaaa -5' |
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| 27641 | 3' | -57.1 | NC_005882.1 | + | 25985 | 0.66 | 0.478943 |
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Target: 5'- aGUa-CACCAUGCCGGCgagGUcuuucaugucgGCGUUc -3' miRNA: 3'- gCAgcGUGGUACGGCCGa--CA-----------CGCAAa -5' |
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| 27641 | 3' | -57.1 | NC_005882.1 | + | 23624 | 0.66 | 0.478943 |
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Target: 5'- gGUCG-GCCAUGCCG-CcGUGCGg-- -3' miRNA: 3'- gCAGCgUGGUACGGCcGaCACGCaaa -5' |
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| 27641 | 3' | -57.1 | NC_005882.1 | + | 12536 | 0.67 | 0.399204 |
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Target: 5'- uGUCGCGgCGgcgGCCGGCgcUGCGg-- -3' miRNA: 3'- gCAGCGUgGUa--CGGCCGacACGCaaa -5' |
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| 27641 | 3' | -57.1 | NC_005882.1 | + | 15086 | 0.67 | 0.389845 |
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Target: 5'- aGUCGCG-CAUGUCGGCUGacacgccggugcUGCGc-- -3' miRNA: 3'- gCAGCGUgGUACGGCCGAC------------ACGCaaa -5' |
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| 27641 | 3' | -57.1 | NC_005882.1 | + | 25899 | 0.69 | 0.320315 |
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Target: 5'- -uUCGCGuCCAUGCCGGCagcgaccagUGUGCc--- -3' miRNA: 3'- gcAGCGU-GGUACGGCCG---------ACACGcaaa -5' |
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| 27641 | 3' | -57.1 | NC_005882.1 | + | 32161 | 0.71 | 0.240536 |
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Target: 5'- aGUCGCGCCGUGCaGGUUcaggGCGUg- -3' miRNA: 3'- gCAGCGUGGUACGgCCGAca--CGCAaa -5' |
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| 27641 | 3' | -57.1 | NC_005882.1 | + | 5436 | 1.05 | 0.000677 |
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Target: 5'- gCGUCGCACCAUGCCGGCUGUGCGUUUc -3' miRNA: 3'- -GCAGCGUGGUACGGCCGACACGCAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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