Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27641 | 5' | -51.1 | NC_005882.1 | + | 35821 | 0.66 | 0.807605 |
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Target: 5'- gGGAGUGU-UGCgGUCAGCG--UCGCGa -3' miRNA: 3'- aCUUCACGuAUG-CGGUCGCaaAGCGU- -5' |
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| 27641 | 5' | -51.1 | NC_005882.1 | + | 16685 | 0.66 | 0.797327 |
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Target: 5'- aGAAGUGCAgcagGCGCUcguAGaCGUcggCGCu -3' miRNA: 3'- aCUUCACGUa---UGCGG---UC-GCAaa-GCGu -5' |
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| 27641 | 5' | -51.1 | NC_005882.1 | + | 16009 | 0.66 | 0.775112 |
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Target: 5'- -cGGGUGCGUGCGCCgccgcaaGGCGgc-CGUg -3' miRNA: 3'- acUUCACGUAUGCGG-------UCGCaaaGCGu -5' |
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| 27641 | 5' | -51.1 | NC_005882.1 | + | 28261 | 0.67 | 0.754364 |
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Target: 5'- -aAGGUGC---CGCCGGCGgccgCGCAg -3' miRNA: 3'- acUUCACGuauGCGGUCGCaaa-GCGU- -5' |
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| 27641 | 5' | -51.1 | NC_005882.1 | + | 29164 | 0.67 | 0.754364 |
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Target: 5'- cUGAAGaucgGC-UACGCCGGCGcgccguugcUCGCGu -3' miRNA: 3'- -ACUUCa---CGuAUGCGGUCGCaa-------AGCGU- -5' |
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| 27641 | 5' | -51.1 | NC_005882.1 | + | 12012 | 0.68 | 0.697542 |
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Target: 5'- cUGggGcaUGgAUGCGCCAGUcucGUcgUUCGCGg -3' miRNA: 3'- -ACuuC--ACgUAUGCGGUCG---CA--AAGCGU- -5' |
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| 27641 | 5' | -51.1 | NC_005882.1 | + | 5066 | 0.69 | 0.650684 |
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Target: 5'- cGAAGcGCAUgcuGCGCUGGCGUaUCgGCGu -3' miRNA: 3'- aCUUCaCGUA---UGCGGUCGCAaAG-CGU- -5' |
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| 27641 | 5' | -51.1 | NC_005882.1 | + | 27818 | 0.7 | 0.561403 |
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Target: 5'- cGAAGcGCGaGCGCCGcGCGUacaggagcugacgugUUCGCAa -3' miRNA: 3'- aCUUCaCGUaUGCGGU-CGCA---------------AAGCGU- -5' |
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| 27641 | 5' | -51.1 | NC_005882.1 | + | 5471 | 1.1 | 0.001246 |
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Target: 5'- uUGAAGUGCAUACGCCAGCGUUUCGCAa -3' miRNA: 3'- -ACUUCACGUAUGCGGUCGCAAAGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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