Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27645 | 5' | -54.3 | NC_005882.1 | + | 31976 | 0.66 | 0.704577 |
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Target: 5'- uGGaGAAU-CGCUUCGCuaggcAGCCCCguGUCGa -3' miRNA: 3'- -CC-CUUAuGCGAGGCGc----UUGGGG--UAGC- -5' |
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| 27645 | 5' | -54.3 | NC_005882.1 | + | 16538 | 0.66 | 0.671193 |
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Target: 5'- -cGGAUGCGCUgCGCGGaugcuugccgACCagCAUCGg -3' miRNA: 3'- ccCUUAUGCGAgGCGCU----------UGGg-GUAGC- -5' |
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| 27645 | 5' | -54.3 | NC_005882.1 | + | 33090 | 0.66 | 0.671193 |
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Target: 5'- cGGGcgcggcGAUAUGCUgCGCaAGCCCgGUCu -3' miRNA: 3'- -CCC------UUAUGCGAgGCGcUUGGGgUAGc -5' |
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| 27645 | 5' | -54.3 | NC_005882.1 | + | 27861 | 0.66 | 0.671193 |
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Target: 5'- -----cGCGCUUCGCGAGCaggUCGUCGa -3' miRNA: 3'- cccuuaUGCGAGGCGCUUGg--GGUAGC- -5' |
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| 27645 | 5' | -54.3 | NC_005882.1 | + | 4012 | 0.67 | 0.648725 |
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Target: 5'- cGGAAgccCGCUUCGCGcAGCUCCAc-- -3' miRNA: 3'- cCCUUau-GCGAGGCGC-UUGGGGUagc -5' |
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| 27645 | 5' | -54.3 | NC_005882.1 | + | 26765 | 0.68 | 0.559041 |
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Target: 5'- cGGGAUGgGCggCCGCGAACUgCG-CGg -3' miRNA: 3'- cCCUUAUgCGa-GGCGCUUGGgGUaGC- -5' |
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| 27645 | 5' | -54.3 | NC_005882.1 | + | 14221 | 0.71 | 0.385181 |
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Target: 5'- cGGAAUuucuucugguucACGCUCgCGCGGGCCagCGUCGc -3' miRNA: 3'- cCCUUA------------UGCGAG-GCGCUUGGg-GUAGC- -5' |
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| 27645 | 5' | -54.3 | NC_005882.1 | + | 20175 | 0.72 | 0.332862 |
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Target: 5'- cGGAAcGCGCgCUGCGcGCCCgGUCGa -3' miRNA: 3'- cCCUUaUGCGaGGCGCuUGGGgUAGC- -5' |
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| 27645 | 5' | -54.3 | NC_005882.1 | + | 29222 | 0.73 | 0.316621 |
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Target: 5'- --aGAUugGCUUCGaCGAACCgCCGUCGa -3' miRNA: 3'- cccUUAugCGAGGC-GCUUGG-GGUAGC- -5' |
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| 27645 | 5' | -54.3 | NC_005882.1 | + | 7784 | 1.11 | 0.000671 |
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Target: 5'- cGGGAAUACGCUCCGCGAACCCCAUCGc -3' miRNA: 3'- -CCCUUAUGCGAGGCGCUUGGGGUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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