Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27647 | 3' | -64.6 | NC_005882.1 | + | 33031 | 0.7 | 0.088986 |
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Target: 5'- uAGCGCGUgcgGCCGCCGagcugCUUCGCCuGCUu -3' miRNA: 3'- -UUGUGCGa--CGGCGGCg----GAGGCGG-CGA- -5' |
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| 27647 | 3' | -64.6 | NC_005882.1 | + | 14197 | 0.71 | 0.081784 |
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Target: 5'- aGGCACGCgcggaagcgGCCGCCGCggCCGUCGaCa -3' miRNA: 3'- -UUGUGCGa--------CGGCGGCGgaGGCGGC-Ga -5' |
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| 27647 | 3' | -64.6 | NC_005882.1 | + | 14262 | 0.71 | 0.081784 |
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Target: 5'- cGGC-CGCgGCgGCCGCUUCCGCgCGUg -3' miRNA: 3'- -UUGuGCGaCGgCGGCGGAGGCG-GCGa -5' |
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| 27647 | 3' | -64.6 | NC_005882.1 | + | 28716 | 0.72 | 0.067084 |
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Target: 5'- uAGCGCGCUGCuCGCCGUCgagagcuggcCCGCgGCc -3' miRNA: 3'- -UUGUGCGACG-GCGGCGGa---------GGCGgCGa -5' |
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| 27647 | 3' | -64.6 | NC_005882.1 | + | 21350 | 0.72 | 0.063375 |
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Target: 5'- gAugACGUcGCCgGCCGCCgUCGCCGCa -3' miRNA: 3'- -UugUGCGaCGG-CGGCGGaGGCGGCGa -5' |
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| 27647 | 3' | -64.6 | NC_005882.1 | + | 27663 | 0.72 | 0.058179 |
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Target: 5'- gGACGcCGCgGCCGCgGCCUgCGUCGCc -3' miRNA: 3'- -UUGU-GCGaCGGCGgCGGAgGCGGCGa -5' |
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| 27647 | 3' | -64.6 | NC_005882.1 | + | 16974 | 0.73 | 0.047612 |
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Target: 5'- gGACGaGCgGCCGCUGCCggugUCCGCCGUUg -3' miRNA: 3'- -UUGUgCGaCGGCGGCGG----AGGCGGCGA- -5' |
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| 27647 | 3' | -64.6 | NC_005882.1 | + | 30075 | 0.79 | 0.017778 |
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Target: 5'- -uCGCGgUGCCGCCGCCUuuGCCGg- -3' miRNA: 3'- uuGUGCgACGGCGGCGGAggCGGCga -5' |
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| 27647 | 3' | -64.6 | NC_005882.1 | + | 10606 | 1.05 | 0.000124 |
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Target: 5'- cAACACGCUGCCGCCGCCUCCGCCGCUg -3' miRNA: 3'- -UUGUGCGACGGCGGCGGAGGCGGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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