Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27647 | 5' | -47.6 | NC_005882.1 | + | 10643 | 1.06 | 0.004986 |
Target: 5'- gAUAACAGCAAUGGGAACGACCAAUCUu -3' miRNA: 3'- -UAUUGUCGUUACCCUUGCUGGUUAGA- -5' |
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27647 | 5' | -47.6 | NC_005882.1 | + | 32660 | 0.76 | 0.464454 |
Target: 5'- uAUGGCGGCAAUGGauuguGGGCGGCCuAUCg -3' miRNA: 3'- -UAUUGUCGUUACC-----CUUGCUGGuUAGa -5' |
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27647 | 5' | -47.6 | NC_005882.1 | + | 15423 | 0.68 | 0.888518 |
Target: 5'- -cGAC-GCGcgGGGcAACGGCCAAUa- -3' miRNA: 3'- uaUUGuCGUuaCCC-UUGCUGGUUAga -5' |
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27647 | 5' | -47.6 | NC_005882.1 | + | 29661 | 0.67 | 0.896445 |
Target: 5'- cGUGACGGguGacuccgUGGGcgucguCGGCCAGUCUg -3' miRNA: 3'- -UAUUGUCguU------ACCCuu----GCUGGUUAGA- -5' |
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27647 | 5' | -47.6 | NC_005882.1 | + | 11079 | 0.66 | 0.937042 |
Target: 5'- ----gGGCGAUGGGAuacGCGGaCGAUCa -3' miRNA: 3'- uauugUCGUUACCCU---UGCUgGUUAGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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