Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27656 | 5' | -59.7 | NC_005882.1 | + | 14738 | 0.67 | 0.31971 |
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Target: 5'- uGCCG-CGCAGguu-GGACGGGCg--- -3' miRNA: 3'- -CGGCuGCGUCagguCCUGCCCGagau -5' |
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| 27656 | 5' | -59.7 | NC_005882.1 | + | 27148 | 0.67 | 0.319709 |
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Target: 5'- gGCCuGAUGCuGgCCGGGcgcGCGGuGCUCUGg -3' miRNA: 3'- -CGG-CUGCGuCaGGUCC---UGCC-CGAGAU- -5' |
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| 27656 | 5' | -59.7 | NC_005882.1 | + | 14579 | 0.68 | 0.261361 |
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Target: 5'- aGUCGGCGCAGUCCcGGAUGaaCUCc- -3' miRNA: 3'- -CGGCUGCGUCAGGuCCUGCccGAGau -5' |
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| 27656 | 5' | -59.7 | NC_005882.1 | + | 26788 | 0.69 | 0.217686 |
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Target: 5'- aGCCGAccacgcCGCGGaucggCCGGGAUGGGCg--- -3' miRNA: 3'- -CGGCU------GCGUCa----GGUCCUGCCCGagau -5' |
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| 27656 | 5' | -59.7 | NC_005882.1 | + | 16704 | 0.7 | 0.211982 |
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Target: 5'- cGUCGACGCGcgcGUCCgcgcAGGcCGGGCUUg- -3' miRNA: 3'- -CGGCUGCGU---CAGG----UCCuGCCCGAGau -5' |
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| 27656 | 5' | -59.7 | NC_005882.1 | + | 27707 | 0.79 | 0.04334 |
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Target: 5'- cGCCGACGauuaCGGUCCAGGGC-GGCUCa- -3' miRNA: 3'- -CGGCUGC----GUCAGGUCCUGcCCGAGau -5' |
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| 27656 | 5' | -59.7 | NC_005882.1 | + | 14902 | 1.08 | 0.000238 |
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Target: 5'- cGCCGACGCAGUCCAGGACGGGCUCUAc -3' miRNA: 3'- -CGGCUGCGUCAGGUCCUGCCCGAGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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