Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27657 | 3' | -56.1 | NC_005882.1 | + | 3862 | 0.67 | 0.459161 |
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Target: 5'- -gUGCUUGAgcgucgcgaUCGGCagcgCgUGCGCUUCGu -3' miRNA: 3'- ggACGAACU---------AGCCGa---GgGCGCGAAGU- -5' |
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| 27657 | 3' | -56.1 | NC_005882.1 | + | 11507 | 0.68 | 0.439013 |
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Target: 5'- gUCgGCUUGcgCGGCguUCCUGCGaUUCAg -3' miRNA: 3'- -GGaCGAACuaGCCG--AGGGCGCgAAGU- -5' |
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| 27657 | 3' | -56.1 | NC_005882.1 | + | 17214 | 0.68 | 0.41938 |
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Target: 5'- gCUGCagGAaCGGCUugCCCGCGCg--- -3' miRNA: 3'- gGACGaaCUaGCCGA--GGGCGCGaagu -5' |
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| 27657 | 3' | -56.1 | NC_005882.1 | + | 27316 | 0.72 | 0.235458 |
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Target: 5'- aCUGCUUGAUCGGCgUCCaguaGCGgUa-- -3' miRNA: 3'- gGACGAACUAGCCG-AGGg---CGCgAagu -5' |
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| 27657 | 3' | -56.1 | NC_005882.1 | + | 27879 | 0.73 | 0.217197 |
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Target: 5'- uCCUGUacGcgCGGCgCUCGCGCUUCGc -3' miRNA: 3'- -GGACGaaCuaGCCGaGGGCGCGAAGU- -5' |
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| 27657 | 3' | -56.1 | NC_005882.1 | + | 15383 | 1.1 | 0.000378 |
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Target: 5'- aCCUGCUUGAUCGGCUCCCGCGCUUCAc -3' miRNA: 3'- -GGACGAACUAGCCGAGGGCGCGAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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