Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27658 | 5' | -56.1 | NC_005882.1 | + | 11342 | 0.66 | 0.56344 |
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Target: 5'- cGCGUUgcUCGCuGGuuggaUCGACCUGUCg -3' miRNA: 3'- -UGCAGa-AGCG-CCugg--AGCUGGACAGa -5' |
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| 27658 | 5' | -56.1 | NC_005882.1 | + | 34508 | 0.66 | 0.56344 |
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Target: 5'- cCGUCgUUGCGaGCUUCGugCUGUUg -3' miRNA: 3'- uGCAGaAGCGCcUGGAGCugGACAGa -5' |
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| 27658 | 5' | -56.1 | NC_005882.1 | + | 16946 | 0.67 | 0.519896 |
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Target: 5'- uGCGUCagcuuuuggUCGCGGccgGCCuggaucucgUCGACCUGUUc -3' miRNA: 3'- -UGCAGa--------AGCGCC---UGG---------AGCUGGACAGa -5' |
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| 27658 | 5' | -56.1 | NC_005882.1 | + | 34471 | 0.68 | 0.437334 |
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Target: 5'- gGCGUUUgugcaGCGGGCCgUCGGCUcgGUCg -3' miRNA: 3'- -UGCAGAag---CGCCUGG-AGCUGGa-CAGa -5' |
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| 27658 | 5' | -56.1 | NC_005882.1 | + | 19442 | 0.68 | 0.437334 |
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Target: 5'- cGCGaCUUCGCGGAUcgaCUCGAUUUGcCa -3' miRNA: 3'- -UGCaGAAGCGCCUG---GAGCUGGACaGa -5' |
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| 27658 | 5' | -56.1 | NC_005882.1 | + | 15589 | 0.68 | 0.427543 |
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Target: 5'- gACGUgcUCGCGGACCUgCGcGCCguacagGUCa -3' miRNA: 3'- -UGCAgaAGCGCCUGGA-GC-UGGa-----CAGa -5' |
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| 27658 | 5' | -56.1 | NC_005882.1 | + | 31454 | 0.69 | 0.398965 |
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Target: 5'- uCGUCUUCGCGGGCUgcgcCGGCagccgcGUCa -3' miRNA: 3'- uGCAGAAGCGCCUGGa---GCUGga----CAGa -5' |
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| 27658 | 5' | -56.1 | NC_005882.1 | + | 29147 | 0.7 | 0.33724 |
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Target: 5'- gUGUCUUCGagcacGACCUCGAUCUcGUCg -3' miRNA: 3'- uGCAGAAGCgc---CUGGAGCUGGA-CAGa -5' |
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| 27658 | 5' | -56.1 | NC_005882.1 | + | 16103 | 1.07 | 0.000774 |
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Target: 5'- gACGUCUUCGCGGACCUCGACCUGUCUc -3' miRNA: 3'- -UGCAGAAGCGCCUGGAGCUGGACAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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