Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2766 | 3' | -54.7 | NC_001491.2 | + | 116162 | 0.67 | 0.903298 |
Target: 5'- gGCUCc-GCGGCCGCggagGGGagccUUC-AGACc -3' miRNA: 3'- -CGAGauCGUCGGCGa---CCC----AAGaUCUG- -5' |
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2766 | 3' | -54.7 | NC_001491.2 | + | 147155 | 0.68 | 0.844454 |
Target: 5'- cGCUCUGGgAG-UGCUGGGg---GGACu -3' miRNA: 3'- -CGAGAUCgUCgGCGACCCaagaUCUG- -5' |
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2766 | 3' | -54.7 | NC_001491.2 | + | 14930 | 0.69 | 0.791736 |
Target: 5'- cGC-CUGGCAGCacugCGCUGGGgga-GGGCg -3' miRNA: 3'- -CGaGAUCGUCG----GCGACCCaagaUCUG- -5' |
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2766 | 3' | -54.7 | NC_001491.2 | + | 126629 | 0.69 | 0.791736 |
Target: 5'- cGCUaucGCAGUCGCUGuaUUCUAGAUc -3' miRNA: 3'- -CGAgauCGUCGGCGACccAAGAUCUG- -5' |
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2766 | 3' | -54.7 | NC_001491.2 | + | 54477 | 0.7 | 0.772866 |
Target: 5'- cCUCcAGCAGCCGCcugggaGGGUucaUCUGcGACa -3' miRNA: 3'- cGAGaUCGUCGGCGa-----CCCA---AGAU-CUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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