Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27663 | 5' | -55.9 | NC_005882.1 | + | 15622 | 0.66 | 0.540905 |
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Target: 5'- aUCAAGG--GCGUGCCgcgcaucccGGACGUCCg- -3' miRNA: 3'- -GGUUCCagUGCGCGGa--------CCUGUAGGac -5' |
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| 27663 | 5' | -55.9 | NC_005882.1 | + | 3747 | 0.66 | 0.540905 |
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Target: 5'- cUCGAcGGcC-CGCGCCUGGACGaauaccUCCg- -3' miRNA: 3'- -GGUU-CCaGuGCGCGGACCUGU------AGGac -5' |
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| 27663 | 5' | -55.9 | NC_005882.1 | + | 14390 | 0.67 | 0.530011 |
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Target: 5'- gUCAAGGUCugcgagaaguCGCGCCgcguuGGCuuGUCCUGg -3' miRNA: 3'- -GGUUCCAGu---------GCGCGGac---CUG--UAGGAC- -5' |
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| 27663 | 5' | -55.9 | NC_005882.1 | + | 5647 | 0.67 | 0.519198 |
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Target: 5'- gCAcAGGUUGCGCGUCaUGGACG-CCg- -3' miRNA: 3'- gGU-UCCAGUGCGCGG-ACCUGUaGGac -5' |
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| 27663 | 5' | -55.9 | NC_005882.1 | + | 32535 | 0.67 | 0.519198 |
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Target: 5'- aCAAGGgCACGC-CCUGGGCggUCa- -3' miRNA: 3'- gGUUCCaGUGCGcGGACCUGuaGGac -5' |
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| 27663 | 5' | -55.9 | NC_005882.1 | + | 18727 | 0.68 | 0.436359 |
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Target: 5'- gCUAucGUUGCGCGCCUcgGGugGUCCg- -3' miRNA: 3'- -GGUucCAGUGCGCGGA--CCugUAGGac -5' |
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| 27663 | 5' | -55.9 | NC_005882.1 | + | 24074 | 0.69 | 0.397885 |
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Target: 5'- gCAAGGUCGaGCuGCC-GGGCggCCUGg -3' miRNA: 3'- gGUUCCAGUgCG-CGGaCCUGuaGGAC- -5' |
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| 27663 | 5' | -55.9 | NC_005882.1 | + | 23001 | 0.69 | 0.388612 |
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Target: 5'- aCgAAGGUCACG-GCCgcGGACAUCa-- -3' miRNA: 3'- -GgUUCCAGUGCgCGGa-CCUGUAGgac -5' |
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| 27663 | 5' | -55.9 | NC_005882.1 | + | 17411 | 1.09 | 0.00052 |
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Target: 5'- gCCAAGGUCACGCGCCUGGACAUCCUGc -3' miRNA: 3'- -GGUUCCAGUGCGCGGACCUGUAGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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