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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27664 | 3' | -52.1 | NC_005882.1 | + | 28364 | 0.66 | 0.793937 |
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Target: 5'- aGUCcgCGaUGCGcguGGCcgucgacgcgGUCAAGGUCGu -3' miRNA: 3'- cUAGuaGC-ACGCu--CCG----------UAGUUCCAGC- -5' |
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| 27664 | 3' | -52.1 | NC_005882.1 | + | 23430 | 0.66 | 0.751499 |
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Target: 5'- -cUCGUCG-GCGucacGGGCGUCGAGcggccgcuGUCGg -3' miRNA: 3'- cuAGUAGCaCGC----UCCGUAGUUC--------CAGC- -5' |
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| 27664 | 3' | -52.1 | NC_005882.1 | + | 6637 | 0.67 | 0.698938 |
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Target: 5'- aGUCGUCGUGCGGcgcgccgugcgucucGGCGUgcugcgcCAAGGUUu -3' miRNA: 3'- cUAGUAGCACGCU---------------CCGUA-------GUUCCAGc -5' |
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| 27664 | 3' | -52.1 | NC_005882.1 | + | 5324 | 0.79 | 0.168785 |
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Target: 5'- uGUCGUCgGUGCcGGGCGUCAuGGUCGg -3' miRNA: 3'- cUAGUAG-CACGcUCCGUAGUuCCAGC- -5' |
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| 27664 | 3' | -52.1 | NC_005882.1 | + | 17853 | 1.1 | 0.00108 |
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Target: 5'- aGAUCAUCGUGCGAGGCAUCAAGGUCGa -3' miRNA: 3'- -CUAGUAGCACGCUCCGUAGUUCCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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