Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
27666 | 3' | -54.6 | NC_005882.1 | + | 2090 | 0.66 | 0.600607 |
Target: 5'- cGAGCA-CGCGGcCGGUGuugaucuGGCCuUCc -3' miRNA: 3'- uCUUGUaGCGCCaGCCGCu------UCGGuAG- -5' |
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27666 | 3' | -54.6 | NC_005882.1 | + | 21221 | 0.66 | 0.612001 |
Target: 5'- cGAGuCGgaCGCGGcCGGCcAAGCCAUUc -3' miRNA: 3'- uCUU-GUa-GCGCCaGCCGcUUCGGUAG- -5' |
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27666 | 3' | -54.6 | NC_005882.1 | + | 9964 | 0.66 | 0.612001 |
Target: 5'- gAGAACAucauuucccgUCGC-GUCGGCGAucuGCCc-- -3' miRNA: 3'- -UCUUGU----------AGCGcCAGCCGCUu--CGGuag -5' |
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27666 | 3' | -54.6 | NC_005882.1 | + | 32469 | 0.66 | 0.623415 |
Target: 5'- aGGAACGgcagUGCGGaugCGuCGAcGCCAUCg -3' miRNA: 3'- -UCUUGUa---GCGCCa--GCcGCUuCGGUAG- -5' |
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27666 | 3' | -54.6 | NC_005882.1 | + | 17547 | 0.66 | 0.634837 |
Target: 5'- aGGAGCAcgUCGaccaGGauacCGGCGAAGUCAa- -3' miRNA: 3'- -UCUUGU--AGCg---CCa---GCCGCUUCGGUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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