Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27666 | 3' | -54.6 | NC_005882.1 | + | 11638 | 0.67 | 0.533176 |
Target: 5'- aGGAugAUCGCGG-CGaCGAgccAGCuCGUCa -3' miRNA: 3'- -UCUugUAGCGCCaGCcGCU---UCG-GUAG- -5' |
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27666 | 3' | -54.6 | NC_005882.1 | + | 15634 | 0.67 | 0.533176 |
Target: 5'- cGAGCA-CGuCGGcauccuUCGG-GAGGCCGUCg -3' miRNA: 3'- uCUUGUaGC-GCC------AGCCgCUUCGGUAG- -5' |
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27666 | 3' | -54.6 | NC_005882.1 | + | 5312 | 0.72 | 0.288093 |
Target: 5'- cGGGCGUCaUGGUCGGCGAgaAGCUgaugAUCa -3' miRNA: 3'- uCUUGUAGcGCCAGCCGCU--UCGG----UAG- -5' |
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27666 | 3' | -54.6 | NC_005882.1 | + | 25139 | 0.8 | 0.090752 |
Target: 5'- ---uCAUCGCGG-CGGCGAucaaGGCCAUCg -3' miRNA: 3'- ucuuGUAGCGCCaGCCGCU----UCGGUAG- -5' |
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27666 | 3' | -54.6 | NC_005882.1 | + | 32469 | 0.66 | 0.623415 |
Target: 5'- aGGAACGgcagUGCGGaugCGuCGAcGCCAUCg -3' miRNA: 3'- -UCUUGUa---GCGCCa--GCcGCUuCGGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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