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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27667 | 5' | -53.4 | NC_005882.1 | + | 12532 | 0.66 | 0.728749 |
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Target: 5'- aGUGUGucgcggcggcGGCCGgCgCUGCGGCgaucgGCGCa -3' miRNA: 3'- gCAUAC----------UCGGCaG-GAUGCCGa----UGUGc -5' |
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| 27667 | 5' | -53.4 | NC_005882.1 | + | 19943 | 0.66 | 0.728749 |
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Target: 5'- uGUAUGcacgaaugcGGCCGUCCagcuCGGC-GCGCu -3' miRNA: 3'- gCAUAC---------UCGGCAGGau--GCCGaUGUGc -5' |
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| 27667 | 5' | -53.4 | NC_005882.1 | + | 16150 | 0.66 | 0.706613 |
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Target: 5'- ----gGAcGCCGUCCUguACGGCUAUcaGCc -3' miRNA: 3'- gcauaCU-CGGCAGGA--UGCCGAUG--UGc -5' |
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| 27667 | 5' | -53.4 | NC_005882.1 | + | 31646 | 0.66 | 0.695421 |
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Target: 5'- cCGgcUGccAGCCGUucccgaucgagCCUGCGGUUucGCACGu -3' miRNA: 3'- -GCauAC--UCGGCA-----------GGAUGCCGA--UGUGC- -5' |
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| 27667 | 5' | -53.4 | NC_005882.1 | + | 34404 | 0.67 | 0.672852 |
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Target: 5'- gCGUgAUGAGCgGUCCUggAUGGaagACGCa -3' miRNA: 3'- -GCA-UACUCGgCAGGA--UGCCga-UGUGc -5' |
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| 27667 | 5' | -53.4 | NC_005882.1 | + | 20827 | 0.67 | 0.650117 |
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Target: 5'- gCGUGU-AGCCGUaggACGGCUcAUACGg -3' miRNA: 3'- -GCAUAcUCGGCAggaUGCCGA-UGUGC- -5' |
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| 27667 | 5' | -53.4 | NC_005882.1 | + | 3280 | 0.68 | 0.615911 |
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Target: 5'- ----cGaAGUCGUCCgACGuGCUGCGCGa -3' miRNA: 3'- gcauaC-UCGGCAGGaUGC-CGAUGUGC- -5' |
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| 27667 | 5' | -53.4 | NC_005882.1 | + | 20765 | 1.09 | 0.001076 |
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Target: 5'- cCGUAUGAGCCGUCCUACGGCUACACGc -3' miRNA: 3'- -GCAUACUCGGCAGGAUGCCGAUGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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