Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27676 | 3' | -63.8 | NC_005882.1 | + | 28603 | 0.66 | 0.197145 |
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Target: 5'- cAGuauuGCUGGCGcgcaggauGCCGGCCGCCUc -3' miRNA: 3'- uUCuccuCGACCGC--------CGGCCGGUGGAc -5' |
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| 27676 | 3' | -63.8 | NC_005882.1 | + | 3245 | 0.66 | 0.191968 |
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Target: 5'- -cGAGGuacgGGCcgaucUGGaucguCGGCCGGCCGCCg- -3' miRNA: 3'- uuCUCC----UCG-----ACC-----GCCGGCCGGUGGac -5' |
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| 27676 | 3' | -63.8 | NC_005882.1 | + | 13721 | 0.66 | 0.191968 |
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Target: 5'- cGAGccGGGCaucGcGCGGCCGGCCAaguaCUGg -3' miRNA: 3'- -UUCucCUCGa--C-CGCCGGCCGGUg---GAC- -5' |
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| 27676 | 3' | -63.8 | NC_005882.1 | + | 6458 | 0.66 | 0.186911 |
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Target: 5'- --cAGGcAGCgcgcGCGGCCGGCCACg-- -3' miRNA: 3'- uucUCC-UCGac--CGCCGGCCGGUGgac -5' |
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| 27676 | 3' | -63.8 | NC_005882.1 | + | 3374 | 0.66 | 0.181971 |
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Target: 5'- --cGGGcGCUGcGCGGCCugGGCUACCg- -3' miRNA: 3'- uucUCCuCGAC-CGCCGG--CCGGUGGac -5' |
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| 27676 | 3' | -63.8 | NC_005882.1 | + | 17864 | 0.67 | 0.163353 |
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Target: 5'- -cGAGGcaucaaGGUcgaGGCGGCCGGCgACCg- -3' miRNA: 3'- uuCUCC------UCGa--CCGCCGGCCGgUGGac -5' |
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| 27676 | 3' | -63.8 | NC_005882.1 | + | 32960 | 0.68 | 0.142501 |
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Target: 5'- cAAGcAGGcgaagcAGCUcGGCGGCCGcacgcGCUACCUGa -3' miRNA: 3'- -UUC-UCC------UCGA-CCGCCGGC-----CGGUGGAC- -5' |
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| 27676 | 3' | -63.8 | NC_005882.1 | + | 9397 | 0.68 | 0.138252 |
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Target: 5'- -cGAGGuAGCUGGCGuCCGGCgaacgcuCGCCUu -3' miRNA: 3'- uuCUCC-UCGACCGCcGGCCG-------GUGGAc -5' |
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| 27676 | 3' | -63.8 | NC_005882.1 | + | 23870 | 0.76 | 0.033225 |
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Target: 5'- -uGAuGAGCaGGUGGCCGGCCGCCa- -3' miRNA: 3'- uuCUcCUCGaCCGCCGGCCGGUGGac -5' |
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| 27676 | 3' | -63.8 | NC_005882.1 | + | 23802 | 1.04 | 0.000152 |
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Target: 5'- aAAGAGGAGCUGGCGGCCGGCCACCUGc -3' miRNA: 3'- -UUCUCCUCGACCGCCGGCCGGUGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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