Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27679 | 5' | -60.1 | NC_005882.1 | + | 30008 | 0.66 | 0.319393 |
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Target: 5'- -cGUCCAGGCc-GGCAaaggcGGCGGCaCCg- -3' miRNA: 3'- ucCAGGUCCGcuUCGU-----UCGCCG-GGaa -5' |
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| 27679 | 5' | -60.1 | NC_005882.1 | + | 26707 | 0.66 | 0.311486 |
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Target: 5'- cAGGUCCGuGaGCGAcggcAGCAGcgcGCGGCCa-- -3' miRNA: 3'- -UCCAGGU-C-CGCU----UCGUU---CGCCGGgaa -5' |
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| 27679 | 5' | -60.1 | NC_005882.1 | + | 4524 | 0.66 | 0.288659 |
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Target: 5'- cAGGUCgAGGUcgucGAGGCugcGCGGCCa-- -3' miRNA: 3'- -UCCAGgUCCG----CUUCGuu-CGCCGGgaa -5' |
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| 27679 | 5' | -60.1 | NC_005882.1 | + | 23435 | 0.66 | 0.288659 |
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Target: 5'- cGGcGUCaCGGGCGu--CGAGCGGCCgCUg -3' miRNA: 3'- -UC-CAG-GUCCGCuucGUUCGCCGG-GAa -5' |
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| 27679 | 5' | -60.1 | NC_005882.1 | + | 26859 | 0.66 | 0.288659 |
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Target: 5'- cGGUCgGGGCGAAGUGGacgcuguuucGCGcGCUCUg -3' miRNA: 3'- uCCAGgUCCGCUUCGUU----------CGC-CGGGAa -5' |
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| 27679 | 5' | -60.1 | NC_005882.1 | + | 25751 | 0.67 | 0.253572 |
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Target: 5'- cAGG-CCGGcuuCGAAGguGGCGGCCUg- -3' miRNA: 3'- -UCCaGGUCc--GCUUCguUCGCCGGGaa -5' |
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| 27679 | 5' | -60.1 | NC_005882.1 | + | 7234 | 0.7 | 0.164376 |
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Target: 5'- cAGGUCCGGGuCGguGCAAuCGGCCg-- -3' miRNA: 3'- -UCCAGGUCC-GCuuCGUUcGCCGGgaa -5' |
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| 27679 | 5' | -60.1 | NC_005882.1 | + | 32949 | 0.74 | 0.082777 |
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Target: 5'- gGGGUUucgaacaagCAGGCGAAGCAgcucGGCGGCCg-- -3' miRNA: 3'- -UCCAG---------GUCCGCUUCGU----UCGCCGGgaa -5' |
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| 27679 | 5' | -60.1 | NC_005882.1 | + | 25542 | 1.05 | 0.000272 |
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Target: 5'- cAGGUCCAGGCGAAGCAAGCGGCCCUUg -3' miRNA: 3'- -UCCAGGUCCGCUUCGUUCGCCGGGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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