Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
2768 | 3' | -63.1 | NC_001491.2 | + | 95446 | 0.66 | 0.584313 |
Target: 5'- uGCGcCCCGCCac-GAGuCUGUCCAUCAg -3' miRNA: 3'- -UGCaGGGCGGagaCUC-GGCGGGUGGU- -5' |
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2768 | 3' | -63.1 | NC_001491.2 | + | 60146 | 0.66 | 0.584313 |
Target: 5'- cGCGcCUgGCCUCgagggcccugaGAGCCGCuuCCGCCGc -3' miRNA: 3'- -UGCaGGgCGGAGa----------CUCGGCG--GGUGGU- -5' |
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2768 | 3' | -63.1 | NC_001491.2 | + | 116975 | 0.67 | 0.507976 |
Target: 5'- cGCGg-CCGCCUCgguggccaUGAGCCG-CCGCUAc -3' miRNA: 3'- -UGCagGGCGGAG--------ACUCGGCgGGUGGU- -5' |
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2768 | 3' | -63.1 | NC_001491.2 | + | 147804 | 0.67 | 0.507976 |
Target: 5'- gGCGgcccUCCGUCUCagcGGGCCGCuggCCGCCAg -3' miRNA: 3'- -UGCa---GGGCGGAGa--CUCGGCG---GGUGGU- -5' |
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2768 | 3' | -63.1 | NC_001491.2 | + | 141770 | 0.67 | 0.497787 |
Target: 5'- gACGgaUCCUGCCgcaUGGcccagcgggggacGCCGUCCACCAg -3' miRNA: 3'- -UGC--AGGGCGGag-ACU-------------CGGCGGGUGGU- -5' |
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2768 | 3' | -63.1 | NC_001491.2 | + | 68883 | 0.68 | 0.436175 |
Target: 5'- uGCGgCCCGCCauaGAGCCGCCgCAgCGg -3' miRNA: 3'- -UGCaGGGCGGagaCUCGGCGG-GUgGU- -5' |
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2768 | 3' | -63.1 | NC_001491.2 | + | 118754 | 0.69 | 0.386338 |
Target: 5'- aGCGgcgCCCGUCgUCcGAG-CGCCCGCCGc -3' miRNA: 3'- -UGCa--GGGCGG-AGaCUCgGCGGGUGGU- -5' |
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2768 | 3' | -63.1 | NC_001491.2 | + | 52188 | 0.7 | 0.362868 |
Target: 5'- uCGUUaugaCUGCUgagCUGAGCCGCUCACCc -3' miRNA: 3'- uGCAG----GGCGGa--GACUCGGCGGGUGGu -5' |
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2768 | 3' | -63.1 | NC_001491.2 | + | 79935 | 0.7 | 0.34778 |
Target: 5'- cGCGUCgUGUCcCUG-GCCGCCCACg- -3' miRNA: 3'- -UGCAGgGCGGaGACuCGGCGGGUGgu -5' |
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2768 | 3' | -63.1 | NC_001491.2 | + | 125494 | 0.7 | 0.347038 |
Target: 5'- cCGcCCCGCCcCgggGAcccgcgggcgcccGCCGCCCGCCGg -3' miRNA: 3'- uGCaGGGCGGaGa--CU-------------CGGCGGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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