Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27681 | 3' | -58.2 | NC_005882.1 | + | 19207 | 0.66 | 0.450008 |
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Target: 5'- aGCCGccGGCccUCAAGGGCCugcGcGACGu -3' miRNA: 3'- cCGGUucCCGu-AGUUCCCGGu--C-CUGC- -5' |
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| 27681 | 3' | -58.2 | NC_005882.1 | + | 30833 | 0.66 | 0.430323 |
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Target: 5'- cGGCCcuuaaccgcGAaGGCGUUguuGAGGGCguGGAUGa -3' miRNA: 3'- -CCGG---------UUcCCGUAG---UUCCCGguCCUGC- -5' |
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| 27681 | 3' | -58.2 | NC_005882.1 | + | 32599 | 0.66 | 0.411152 |
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Target: 5'- uGUCGAGGGCAUCGGuGGcGCCuacuacgauGGGAa- -3' miRNA: 3'- cCGGUUCCCGUAGUU-CC-CGG---------UCCUgc -5' |
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| 27681 | 3' | -58.2 | NC_005882.1 | + | 13071 | 0.68 | 0.348411 |
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Target: 5'- cGGCgGcuGGCGUCGAcgugguGGGCgAGGAUGu -3' miRNA: 3'- -CCGgUucCCGUAGUU------CCCGgUCCUGC- -5' |
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| 27681 | 3' | -58.2 | NC_005882.1 | + | 13830 | 0.68 | 0.323694 |
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Target: 5'- cGUCGAGGGCAacuUCAcGGGCUaucAGGAg- -3' miRNA: 3'- cCGGUUCCCGU---AGUuCCCGG---UCCUgc -5' |
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| 27681 | 3' | -58.2 | NC_005882.1 | + | 23438 | 0.69 | 0.271187 |
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Target: 5'- cGUCAcGGGCGUCGAGcGGCCGcugucGGCGa -3' miRNA: 3'- cCGGUuCCCGUAGUUC-CCGGUc----CUGC- -5' |
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| 27681 | 3' | -58.2 | NC_005882.1 | + | 13022 | 0.7 | 0.250869 |
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Target: 5'- cGGaauCAAGGGCGUCAucggcgcGGGCCGGcGCa -3' miRNA: 3'- -CCg--GUUCCCGUAGUu------CCCGGUCcUGc -5' |
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| 27681 | 3' | -58.2 | NC_005882.1 | + | 25619 | 0.71 | 0.208339 |
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Target: 5'- cGCCAGccGGGUcgCAAGGGCCGcuugcuucgccuGGACc -3' miRNA: 3'- cCGGUU--CCCGuaGUUCCCGGU------------CCUGc -5' |
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| 27681 | 3' | -58.2 | NC_005882.1 | + | 26088 | 1.1 | 0.00024 |
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Target: 5'- cGGCCAAGGGCAUCAAGGGCCAGGACGc -3' miRNA: 3'- -CCGGUUCCCGUAGUUCCCGGUCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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