Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27681 | 5' | -57.9 | NC_005882.1 | + | 11661 | 0.7 | 0.248289 |
Target: 5'- aGUC-UCGUCAGCCUCG----CGGCCGu -3' miRNA: 3'- -UAGuAGCGGUCGGAGCuucaGCCGGC- -5' |
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27681 | 5' | -57.9 | NC_005882.1 | + | 25752 | 0.72 | 0.195047 |
Target: 5'- -gCAg-GCCGGCUUCGAAGguggCGGCCu -3' miRNA: 3'- uaGUagCGGUCGGAGCUUCa---GCCGGc -5' |
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27681 | 5' | -57.9 | NC_005882.1 | + | 31532 | 0.72 | 0.169991 |
Target: 5'- cUCGUCGCCGGCCaguugucgaUCGAcGU-GGCCGc -3' miRNA: 3'- uAGUAGCGGUCGG---------AGCUuCAgCCGGC- -5' |
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27681 | 5' | -57.9 | NC_005882.1 | + | 31270 | 0.74 | 0.128374 |
Target: 5'- cGUC-UUGCCAgugcgcGCCUCGuAGUCGGCCa -3' miRNA: 3'- -UAGuAGCGGU------CGGAGCuUCAGCCGGc -5' |
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27681 | 5' | -57.9 | NC_005882.1 | + | 26125 | 1.06 | 0.000506 |
Target: 5'- gAUCAUCGCCAGCCUCGAAGUCGGCCGc -3' miRNA: 3'- -UAGUAGCGGUCGGAGCUUCAGCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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