miRNA: 3'- cUGCgGAUGUUcuuCCGGCCGCAGCU- -5'
miRNA: 3'- cUGCGGAUGuuCUUCCGGC-CGCaGCU- -5'
miRNA: 3'- -CUGCGG-AUGUUCUUcCGGCCGCaGCU- -5'
miRNA: 3'- -CUGCGGAUGUUCUUCCGGCCGCAGCU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27682 | 3' | -56.4 | NC_005882.1 | + | 13079 | 0.72 | 0.245706 |
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Target: 5'- cGCGgCUGCGGc--GGCUGGCGUCGAc -3' miRNA: 3'- cUGCgGAUGUUcuuCCGGCCGCAGCU- -5' |
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| 27682 | 3' | -56.4 | NC_005882.1 | + | 13787 | 0.76 | 0.116077 |
|
Target: 5'- aACGCCUGCc-GAAGGCCGuGCG-CGAa -3' miRNA: 3'- cUGCGGAUGuuCUUCCGGC-CGCaGCU- -5' |
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| 27682 | 3' | -56.4 | NC_005882.1 | + | 32042 | 0.77 | 0.112783 |
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Target: 5'- uGACGCUgUACAAGGAuGCCGGCGcCGAc -3' miRNA: 3'- -CUGCGG-AUGUUCUUcCGGCCGCaGCU- -5' |
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| 27682 | 3' | -56.4 | NC_005882.1 | + | 26230 | 1.09 | 0.000484 |
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Target: 5'- cGACGCCUACAAGAAGGCCGGCGUCGAc -3' miRNA: 3'- -CUGCGGAUGUUCUUCCGGCCGCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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