Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27690 | 5' | -57.5 | NC_005882.1 | + | 3845 | 0.66 | 0.539401 |
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Target: 5'- aUCCUGAUCGGCCAGccggagcugaaggucAgcugUCCGAGcGCa -3' miRNA: 3'- -GGGACUGGCUGGUC---------------Uaa--GGGCUC-CGc -5' |
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| 27690 | 5' | -57.5 | NC_005882.1 | + | 7422 | 0.66 | 0.524511 |
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Target: 5'- aCCgUGACCGACgCcGAUgUUCCG-GGCa -3' miRNA: 3'- -GGgACUGGCUG-GuCUA-AGGGCuCCGc -5' |
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| 27690 | 5' | -57.5 | NC_005882.1 | + | 13977 | 0.66 | 0.50769 |
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Target: 5'- aUCCUGACCGAaggcgcagcggacgaUCAGGacgcccgCuCCGAGGCu -3' miRNA: 3'- -GGGACUGGCU---------------GGUCUaa-----G-GGCUCCGc -5' |
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| 27690 | 5' | -57.5 | NC_005882.1 | + | 18650 | 0.67 | 0.462699 |
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Target: 5'- gCUCUGcgcGCCu-CCGGAccaCCCGAGGCGc -3' miRNA: 3'- -GGGAC---UGGcuGGUCUaa-GGGCUCCGC- -5' |
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| 27690 | 5' | -57.5 | NC_005882.1 | + | 25582 | 0.68 | 0.377799 |
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Target: 5'- aCCUGAUCGAuuguCCGG----CCGAGGCGg -3' miRNA: 3'- gGGACUGGCU----GGUCuaagGGCUCCGC- -5' |
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| 27690 | 5' | -57.5 | NC_005882.1 | + | 33157 | 0.69 | 0.366429 |
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Target: 5'- gCUUGGCCGACCAGuucggcaagccuugCCgCGAGGUc -3' miRNA: 3'- gGGACUGGCUGGUCuaa-----------GG-GCUCCGc -5' |
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| 27690 | 5' | -57.5 | NC_005882.1 | + | 30327 | 0.71 | 0.248176 |
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Target: 5'- gCCUGAUCGGCCAGggUCUUcGGGUa -3' miRNA: 3'- gGGACUGGCUGGUCuaAGGGcUCCGc -5' |
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| 27690 | 5' | -57.5 | NC_005882.1 | + | 30275 | 0.72 | 0.229529 |
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Target: 5'- aCCCUGGCCGAUCAGGcUUgCUGGaccuacgauaacGGCGa -3' miRNA: 3'- -GGGACUGGCUGGUCU-AAgGGCU------------CCGC- -5' |
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| 27690 | 5' | -57.5 | NC_005882.1 | + | 28513 | 1.11 | 0.000319 |
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Target: 5'- uCCCUGACCGACCAGAUUCCCGAGGCGg -3' miRNA: 3'- -GGGACUGGCUGGUCUAAGGGCUCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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