Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 27695 | 3' | -53.9 | NC_005882.1 | + | 21231 | 0.66 | 0.706542 |
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Target: 5'- gCGGcCGGCcaaGCCAuuCCGAAGgcaacCGGcGCa -3' miRNA: 3'- -GCC-GCCGa--UGGUuuGGCUUCa----GCU-CG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 5667 | 0.66 | 0.695398 |
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Target: 5'- aCGcCGuGUUcGCCGAuCCGgcGUCGAGCa -3' miRNA: 3'- -GCcGC-CGA-UGGUUuGGCuuCAGCUCG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 23121 | 0.66 | 0.684189 |
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Target: 5'- cCGGCGuaCUGCacgcaGAACuCGGucucGGUCGAGCu -3' miRNA: 3'- -GCCGCc-GAUGg----UUUG-GCU----UCAGCUCG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 6663 | 0.66 | 0.684189 |
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Target: 5'- uCGGCGuGCUgcGCCAAGguuUCGAAGgCG-GCa -3' miRNA: 3'- -GCCGC-CGA--UGGUUU---GGCUUCaGCuCG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 34527 | 0.66 | 0.717609 |
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Target: 5'- aCGGCccGCUGCaCAAacGCCGucGUugCGAGCu -3' miRNA: 3'- -GCCGc-CGAUG-GUU--UGGCuuCA--GCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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