Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27695 | 3' | -53.9 | NC_005882.1 | + | 21187 | 0.76 | 0.206621 |
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Target: 5'- uGGcCGGCgcACCGGucgacgagcauGCCGAGGUCGAGUc -3' miRNA: 3'- gCC-GCCGa-UGGUU-----------UGGCUUCAGCUCG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 5399 | 0.76 | 0.184999 |
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Target: 5'- uGGCGGUUGCCucGCCGGaaAGUCGGa- -3' miRNA: 3'- gCCGCCGAUGGuuUGGCU--UCAGCUcg -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 17351 | 0.77 | 0.160799 |
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Target: 5'- uCGGCGGCaGCUucaugcaguAGCCGAGGUCGAccGCu -3' miRNA: 3'- -GCCGCCGaUGGu--------UUGGCUUCAGCU--CG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 10269 | 0.77 | 0.160345 |
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Target: 5'- uCGGCGGCUGCCAguAugCGGggaaaacguacgcGGUCG-GCa -3' miRNA: 3'- -GCCGCCGAUGGU--UugGCU-------------UCAGCuCG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 29962 | 1.12 | 0.000562 |
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Target: 5'- gCGGCGGCUACCAAACCGAAGUCGAGCu -3' miRNA: 3'- -GCCGCCGAUGGUUUGGCUUCAGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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