Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27695 | 3' | -53.9 | NC_005882.1 | + | 32977 | 0.74 | 0.256435 |
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Target: 5'- uCGGCGGCcgcacgcgcUACCugAAGgCGcAGGUCGAGCa -3' miRNA: 3'- -GCCGCCG---------AUGG--UUUgGC-UUCAGCUCG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 33090 | 0.67 | 0.65029 |
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Target: 5'- gCGGCaaGGCuUGCCGAACU--GGUCG-GCc -3' miRNA: 3'- -GCCG--CCG-AUGGUUUGGcuUCAGCuCG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 34527 | 0.66 | 0.717609 |
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Target: 5'- aCGGCccGCUGCaCAAacGCCGucGUugCGAGCu -3' miRNA: 3'- -GCCGc-CGAUG-GUU--UGGCuuCA--GCUCG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 35389 | 0.66 | 0.672926 |
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Target: 5'- uGGCaGGcCUACCgGAGCCGAucgaaGGGCa -3' miRNA: 3'- gCCG-CC-GAUGG-UUUGGCUucag-CUCG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 36443 | 0.67 | 0.627582 |
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Target: 5'- aGGCuaucucauGGCUuacaacGCCAGACUGcgagauucGGUCGAGCc -3' miRNA: 3'- gCCG--------CCGA------UGGUUUGGCu-------UCAGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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