Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27695 | 3' | -53.9 | NC_005882.1 | + | 4898 | 0.68 | 0.571066 |
|
Target: 5'- aGGCGGCaaagcagAUCAAgGCCGcuGUCGaAGCu -3' miRNA: 3'- gCCGCCGa------UGGUU-UGGCuuCAGC-UCG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 3364 | 0.67 | 0.616229 |
|
Target: 5'- gCGGCcugGGCUACCGcgugcuGGCCGAAGagaucUCGccGCg -3' miRNA: 3'- -GCCG---CCGAUGGU------UUGGCUUC-----AGCu-CG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 2467 | 0.7 | 0.431573 |
|
Target: 5'- uGGCGGaCgaaGCCAAAaCGAgaucgaaggggcuGGUCGAGCu -3' miRNA: 3'- gCCGCC-Ga--UGGUUUgGCU-------------UCAGCUCG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 1922 | 0.67 | 0.627582 |
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Target: 5'- -cGCGGUUACgCGuuGgCGAuGUCGAGCg -3' miRNA: 3'- gcCGCCGAUG-GUu-UgGCUuCAGCUCG- -5' |
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| 27695 | 3' | -53.9 | NC_005882.1 | + | 1120 | 0.68 | 0.556542 |
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Target: 5'- aGGCGGaugagcuuUUGCCGAGCCuGAGGcccgcgaucagauuUCGAGUa -3' miRNA: 3'- gCCGCC--------GAUGGUUUGG-CUUC--------------AGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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